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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Study of closely related species within the Physalaemus cuvieri group (Anura): contribution of microsatellite markers

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Author(s):
Conte, M. [1, 2] ; Zucchi, M. I. [3] ; Andrade, G. V. [4] ; Souza, A. P. [1] ; Recco-Pimentel, S. M. [2]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, Dept Biol Vegetal, Inst Biol, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Dept Anat Biol Celular & Fisiol & Biofis, Inst Biol, Campinas, SP - Brazil
[3] Agencia Paulista Tecnol Agr, Polo Reg Ctr Sul, Piracicaba, SP - Brazil
[4] Univ Fed Maranhao, Dept Biol, Ctr Ciencias Saude, Sao Luis, MA - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Genetics and Molecular Research; v. 10, n. 3, p. 1434-1444, 2011.
Web of Science Citations: 3
Abstract

Various species of the Physalaemus cuvieri group of frogs are difficult to distinguish morphologically, making molecular analysis an attractive alternative for indentifying members of this group, which is considered to be at risk because of loss of habitat. The genetic structure of natural populations of P. ephippifer and P. albonotatus species was investigated and analyzed, together with that of five previously studied populations of P. cuvieri. Nine microsatellite loci were used in the analyses. The overall G(ST) value (0.46) revealed high genetic variation among the populations, as expected for different species. Bayesian analysis implemented by the STRUCTURE software clustered the seven populations into seven groups (K = 7). All the P. albonotatus and P. ephippifer specimens were grouped into a single cluster, both species showing clear differentiation from P. cuvieri. The different grouping based on these microsatellites of some P. cuvieri individuals from Porto Nacional and from Passo Fundo suggests that they could be a new species, indicating a necessity for taxonomic reevaluation. Despite the intrinsic difficulties in analyzing closely related species, the nine microsatellite loci were found to be adequate for distinguishing these three species of the P. cuvieri group and their populations. (AU)