High performance liquid chromatography analysis of a 4-anilinoquinazoline derivative (PD153035), a specific inhibitor of the epidermal growth factor receptor tyrosine kinase, in rat plasma

Author(s)

Velho, Jesus Antonio ; Marin, Rodrigo Miguel ; Rolim Filho, Luís de Arruda ; Vercesi, Anibal Eugênio ; Rittner, Roberto ; Franchini, Kleber Gomes ; Rocco, Silvana Aparecida ^[7]

• Document type: Journal article
• Source: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 817, n. 2, p. 297-302, Mar. 2005.
• Field of knowledge: Physical Sciences and Mathematics - Chemistry

Abstract

The quinazoline derivative, 4-N-(3′-bromo-phenyl)amino-6,7-dimethoxyquinazoline (PD153035), has recently been identified as a potential drug for the treatment of proliferative disease. Here, we report a sensitive high performance liquid chromatography (HPLC)-based quantitative detection method for measurement of PD153035 levels in rat plasma. Sample pretreatment involved a two-step extraction with chloroform. The analytes were separated on a column packed with OmniSpher C18 material and eluted with acetonitrile–0.1 M ammonium acetate, pH 7.2 (70:30, v/v). The column effluent was monitored by UV detection at 330 nm. A linear response was achieved over the concentration range 0.50–100.00 μM using multilevel calibration with an internal standard. The analytical method inter- and intra-run accuracy and precision were better than ±15%. The lower limit of quantification was 0.50 μM. The method has been applied to study the preclinical pharmacokinetics of this compound in rats. (AU)

• FAPESP's process: 01/11698-1 - Signaling Mechanisms Induced by Mechanical Overload: Role in Myocardial Hypertrophy and Remodeling
• Grantee: Kleber Gomes Franchini
• Support Opportunities: Research Projects - Thematic Grants