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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Astaxanthin addition improves human neutrophils function: in vitro study

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Author(s):
Macedo, Rita C. [1] ; Bolin, Anaysa P. [1] ; Marin, Douglas P. [1] ; Otton, Rosemari [1, 2]
Total Authors: 4
Affiliation:
[1] Cruzeiro do Sul Univ, Postgrad Program, CBS, BR-03342000 Sao Paulo - Brazil
[2] Cruzeiro do Sul Univ, Postgrad Program, Human Movement Sci Inst Phys Act & Sport Sci, ICAFE, BR-01506000 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: EUROPEAN JOURNAL OF NUTRITION; v. 49, n. 8, p. 447-457, DEC 2010.
Web of Science Citations: 35
Abstract

The aim of the present study was to evaluate the in vitro effect of carotenoid astaxanthin (ASTA) on the phagocytic and microbicidal capacities, cytokine release, and reactive oxygen species production in human neutrophils. The following parameters were evaluated: cytotoxic effect of ASTA on human neutrophils viability, phagocytic and microbicidal capacities of neutrophils by using Candida albicans assay, intracellular calcium mobilization (Fura 2-AM fluorescent probe), superoxide anion (lucigenin and DHE probes), hydrogen peroxide (H(2)O(2), phenol red), and nitric oxide (NO center dot) (Griess reagent) production, activities of antioxidant enzymes (total/Mn-SOD, CAT, GPx, and GR), oxidative damages in biomolecules (TBARS assay and carbonyl groups), and cytokine (IL-6 and TNF-alpha) release. Astaxanthin significantly improves neutrophil phagocytic and microbicidal capacity, and increases the intracellular calcium concentration and NO center dot production. Both functional parameters were accompanied by a decrease in superoxide anion and hydrogen peroxide and IL-6 and TNF-alpha production. Oxidative damages in lipids and proteins were significantly decreased after ASTA-treatment. Taken together our results are supportive to a beneficial effect of astaxanthin-treatment on human neutrophils function as demonstrated by increased phagocytic and fungicide capacity as well as by the reduced superoxide anion and hydrogen peroxide production, however, without affecting neutrophils capacity to kill C. albicans. This process appears to be mediated by calcium released from intracellular storages as well as nitric oxide production. (AU)