Alternative promoters in the pst operon of Escherichia coli

Spira, Beny; Aguena, Meire; de Castro Oliveira, Juliana Velasco; Yagil, Ezra

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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Alternative promoters in the pst operon of Escherichia coli

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Author(s):	Spira, Beny ^[1] ; Aguena, Meire ^[1] ; de Castro Oliveira, Juliana Velasco ^[1] ; Yagil, Ezra ^[2] Total Authors: 4
Affiliation:	^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo - Brazil ^[2] Tel Aviv Univ, Dept Biochem, IL-69978 Tel Aviv - Israel Total Affiliations: 2
Document type:	Journal article
Source:	Molecular Genetics and Genomics; v. 284, n. 6, p. 489-498, DEC 2010.
Web of Science Citations:	7
Abstract
The pst operon of Escherichia coli is composed of five genes pstS, pstC, pstA, pstB and phoU, that encode a high-affinity phosphate transport system and a negative regulator of the PHO regulon. Transcription of pst is induced under phosphate shortage and is initiated at the promoter located upstream of the first gene of the operon, pstS. Here, we show by four different technical approaches the existence of additional internal promoters upstream of pstC, pstB and phoU. These promoters are not induced by Pi-limitation and do not possess PHO-box sequences. Plasmids carrying the pst internal genes partially complement chromosomal mutations in their corresponding genes, indicating that they are translated into functional proteins. (AU)

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