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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular Characterization, Distribution, and Dynamics of Hepatitis C Virus Genotypes in Blood Donors in Colombia

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Author(s):
Alvarado Mora, Monica Viviana [1] ; Romano, Camila Malta [2] ; Gomes-Gouvea, Michele Soares [1] ; Fernanda Gutierrez, Maria [3] ; Carrilho, Flair Jose [1] ; Rebello Pinho, Joao Renato [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Fac Med, Dept Gastroenterol, Lab Gastroenterol Hepatol, Sao Paulo Inst Trop Med, Sch Med, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sao Paulo Inst Trop Med, Virol Lab, Dept Infect & Parasit Dis, Sch Med, Sao Paulo - Brazil
[3] Pontificia Javeriana Univ, Virol Lab, Dept Microbiol, Bogota - Colombia
Total Affiliations: 3
Document type: Journal article
Source: Journal of Medical Virology; v. 82, n. 11, p. 1889-1898, NOV 2010.
Web of Science Citations: 14
Abstract

Hepatitis C virus (HCV) is a frequent cause of acute and chronic hepatitis and a leading cause for cirrhosis of the liver and hepatocellular carcinoma. HCV is classified in six major genotypes and more than 70 subtypes. In Colombian blood banks, serum samples were tested for anti-HCV antibodies using a third-generation ELISA. The aim of this study was to characterize the viral sequences in plasma of 184 volunteer blood donors who attended the ``Banco Nacional de Sangre de la Cruz Roja Colombiana,'' Bogota, Colombia. Three different HCV genomic regions were amplified by nested PCR. The first of these was a segment of 180 bp of the 5'UTR region to confirm the previous diagnosis by ELISA. From those that were positive to the 5'UTR region, two further segments were amplified for genotyping and subtyping by phylogenetic analysis: a segment of 380 bp from the NS5B region; and a segment of 391 bp from the E1 region. The distribution of HCV subtypes was: 1b (82.8%), 1a (5.7%), 2a (5.7%), 2b (2.8%), and 3a (2.8%). By applying Bayesian Markov chain Monte Carlo simulation, it was estimated that HCV-1b was introduced into Bogota around 1950. Also, this subtype spread at an exponential rate between about 1970 to about 1990, after which transmission of HCV was reduced by anti-HCV testing of this population. Among Colombian blood donors, HCV genotype 1b is the most frequent genotype, especially in large urban conglomerates such as Bogota, as is the case in other South American countries. J. Med. Virol. 82: 1889-1898, 2010. (C) 2010 Wiley-Liss, Inc. (AU)