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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of 660 and 780 nm Low-Level Laser Therapy on Neuromuscular Recovery After Crush Injury in Rat Sciatic Nerve

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Author(s):
Gigo-Benato, Davilene [1] ; Russo, Thiago Luiz [2] ; Tanaka, Erika Harumi [1] ; Assis, Livia [1] ; Salvini, Tania Fatima [2] ; Parizotto, Nivaldo Antonio [1]
Total Authors: 6
Affiliation:
[1] Fed Univ Sao Carlos UFSCar, Phys Therapy Dept, Thermophototherapy Unit, BR-13565905 Sao Carlos, SP - Brazil
[2] Fed Univ Sao Carlos UFSCar, Phys Therapy Dept, Skeletal Muscle Plast Unit, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Lasers in Surgery and Medicine; v. 42, n. 9, p. 673-682, NOV 2010.
Web of Science Citations: 46
Abstract

Background and Objective: Post-traumatic nerve repair is still a challenge for rehabilitation. It is particularly important to develop clinical protocols to enhance nerve regeneration. The present study investigated the effects of 660 and 780 nm low-level laser therapy (LLLT) using different energy densities (10, 60, and 120 J/cm(2)) on neuromuscular and functional recovery as well as on matrix metalloproteinase(MMP) activity after crush injury in rat sciatic nerve. Materials and Methods: Rats received transcutaneous LLLT irradiation at the lesion site for 10 consecutive days post-injury and were sacrificed 28 days after injury. Both the sciatic nerve and tibialis anterior muscles were analyzed. Nerve analyses consisted of histology (light microscopy) and measurements of myelin, axon, and nerve fiber cross-sectional area (CSA). S-100 labeling was used to identify myelin sheath and Schwann cells. Muscle fiber CSA and zymography were carried out to assess the degree of muscle atrophy and MMP activity, respectively. Statistical significance was set at 5% (P <= 0.05). Results: Six hundred sixty nanometer LLLT either using 10 or 60 J/cm(2) restored muscle fiber, myelin and nerve fiber CSA compared to the normal group (N). Furthermore, it increased MMP-2 activity in nerve and decreased MMP-2 activity in muscle and MMP-9 activity in nerve. In contrast, 780 nm LLLT using 10 J/cm(2) decreased MMP-9 activity in nerve compared to the crush group (CR) and N; it also restored normal levels of myelin and nerve fiber CSA. Both 60 and 120 J/cm(2) decreased MMP-2 activity in muscle compared to CR and N. 780 nm did not prevent muscle fiber atrophy. Functional recovery in the irradiated groups did not differ from the non-irradiated CR. Conclusion: Data suggest that 660 nm LLLT with low (10 J/cm(2)) or moderate (60 J/cm(2)) energy densities is able to accelerate neuromuscular recovery after nerve crush injury in rats. Lasers Surg. Med. 42:673-682, 2010. (C) 2010 Wiley-Liss, Inc. (AU)

FAPESP's process: 08/03499-8 - The effect of anterior cruciate ligament rupture in morphology, gene and protein expressions related to atrophy in the quadriceps femoral muscle of rats
Grantee:Gabriel Borges Delfino
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)