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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

PAR(2) and Temporomandibular Joint Inflammation in the Rat

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Author(s):
Denadai-Souza, A. [1, 2] ; Cenac, N. [3, 2] ; Casatti, C. A. [4] ; de Souza Camara, P. R. [1] ; Yshii, L. M. [1] ; Costa, S. K. P. [1] ; Vergnolle, N. [3, 2] ; Muscara, M. N. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508900 Sao Paulo - Brazil
[2] Ctr Physiopathol Toulouse Purpan, INSERM U563, F-31000 Toulouse - France
[3] Univ Toulouse 3, F-31000 Toulouse - France
[4] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF DENTAL RESEARCH; v. 89, n. 10, p. 1123-1128, OCT 2010.
Web of Science Citations: 10
Abstract

The proteinase-activated receptor 2 (PAR(2)) is a putative therapeutic target for arthritis. We hypothesized that the early pro-inflammatory effects secondary to its activation in the temporomandibular joint (TMJ) are mediated by neurogenic mechanisms. Immunofluorescence analysis revealed a high degree of neurons expressing PAR(2) in retrogradely labeled trigeminal ganglion neurons. Furthermore, PAR(2) immunoreactivity was observed in the lining layer of the TMJ, co-localizing with the neuronal marker PGP9.5 and substance-P-containing peripheral sensory nerve fibers. The intra-articular injection of PAR(2) agonists into the TMJ triggered a dose-dependent increase in plasma extravasation, neutrophil influx, and induction of mechanical allodynia. The pharmacological blockade of natural killer 1 (NK(1)) receptors abolished PAR(2)-induced plasma extravasation and inhibited neutrophil influx and mechanical allodynia. We conclude that PAR(2) activation is proinflammatory in the TMJ, through a neurogenic mechanism involving NK(1) receptors. This suggests that PAR(2) is an important component of innate neuro-immune response in the rat TMJ. (AU)