Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The non-regular orbit: three satellite DNAs in Drosophila martensis (buzzatii complex, repleta group) followed three different evolutionary pathways

Full text
Author(s):
Kuhn, Gustavo C. S. [1] ; Schwarzacher, Trude [2] ; Heslop-Harrison, John S. [2]
Total Authors: 3
Affiliation:
[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Leicester, Dept Biol, Leicester LE2 3TD, Leics - England
Total Affiliations: 2
Document type: Journal article
Source: Molecular Genetics and Genomics; v. 284, n. 4, p. 251-262, OCT 2010.
Web of Science Citations: 4
Abstract

The genome of species from the buzzatii cluster (buzzatii complex, repleta group) is hosted by a number of satellite DNAs (satDNAs) showing contrasting structural characteristics, genomic organization and evolution, such as pBuM-alpha (similar to 190 bp repeats), pBuM-alpha/beta (similar to 370 bp repeats) and the DBC-150 (similar to 150 bp repeats). In the present study, we aimed to investigate the evolution of these three satDNAs by looking for homologous sequences in the genome of the closest outgroup species: Drosophila martensis (buzzatii complex). After PCR, we isolated and sequenced 9 alpha, 8 alpha/beta and 11 DBC-150 sequences from this species. The results were compared to all pBuM and DBC-150 sequences available in literature. After D. martensis split from the buzzatii cluster some 6 Mya, the three satDNAs evolved differently in the genome of D. martensis by: (1) maintenance of a collection of major types of ancestral repeats in the genome (alpha); (2) fixation for a single major type of ancestral repeats (alpha/beta) or (3) fixation for new divergent species-specific repeat types (DBC-150). Curiously, D. seriema and D. martensis, although belonging to different and allopatric clusters, became independently fixed for the same major type of alpha/beta ancestral repeats, illustrating a rare case of parallelism in satDNA evolution. The contrasting pictures illustrate the diversity of evolutionary pathways a satDNA can follow, defining a ``non-regular orbit{''} with outcomes difficult to predict. (AU)

FAPESP's process: 09/08738-3 - Testing and improving concepts on the organization and evolution of satellite DNAs using whole sequenced genomes of Drosophila
Grantee:Gustavo Campos e Silva Kuhn
Support Opportunities: Scholarships in Brazil - Post-Doctoral