Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Astaxanthin prevents in vitro auto-oxidative injury in human lymphocytes

Full text
Author(s):
Bolin, Anaysa P. [1] ; Macedo, Rita C. [1] ; Marin, Douglas P. [1] ; Barros, Marcelo P. [2] ; Otton, Rosemari [1, 2]
Total Authors: 5
Affiliation:
[1] Univ Cruzeiro Sul, Postgrad Program Hlth Sci, Cellular Physiol Lab, CBS, BR-03342000 Sao Paulo - Brazil
[2] Univ Cruzeiro Sul, Postgrad Program Human Movement Sci, Inst Phys Act & Sport Sci, BR-03342000 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CELL BIOLOGY AND TOXICOLOGY; v. 26, n. 5, p. 457-467, OCT 2010.
Web of Science Citations: 31
Abstract

Upon mitogen sensitization, lymphocytes undergo proliferation by oxyradical-based mechanisms. Through continuous resting-restimulation cycles, lymphocytes accumulate auto-induced oxidative lesions which lead to cell dysfunction and limit their viability. Astaxanthin (ASTA) is a nutritional carotenoid that shows notable antioxidant properties. This study aims to evaluate whether the in vitro ASTA treatment can limit oxyradical production and auto-oxidative injury in human lymphocytes. Activated lymphocytes treated with 5 A mu M ASTA showed immediate lower rates of O (2) (aEuro cent a') /H(2)O(2) production whilst NO(aEuro cent) and intracellular Ca(2+) levels were concomitantly enhanced (a parts per thousand currency sign4 h). In long-term treatments (> 24 h), the cytotoxicity test for ASTA showed a sigmoidal dose-response curve (LC50 = 11.67 A +/- 0.42 A mu M), whereas higher activities of superoxide dismutase and catalase in 5 A mu M ASTA-treated lymphocytes were associated to significant lower indexes of oxidative injury. On the other hand, lower proliferative scores of ASTA lymphocytes might be a result of diminished intracellular levels of pivotal redox signaling molecules, such as H(2)O(2). Further studies are necessary to establish the ASTA-dose compensation point between minimizing oxidative damages and allowing efficient redox-mediated immune functions, such as proliferation, adhesion, and oxidative burst. (AU)