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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Riboflavin and photoproducts in MC3T3-E1 differentiation

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Author(s):
Chaves Neto, Antonio Hernandes [1] ; Yano, Claudia Lumy [1] ; Paredes-Gamero, Edgar Julian [2] ; Machado, Daisy [1] ; Justo, Giselle Zenker [2] ; Peppelenbosch, Maikel P. [3] ; Ferreira, Carmen Verissima [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Dept Bioquim, Inst Biol, BR-13083970 Campinas, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Bioquim, Sao Paulo - Brazil
[3] Univ Groningen, Univ Med Ctr Groningen, Dept Cell Biol, NL-9713 AV Groningen - Netherlands
Total Affiliations: 3
Document type: Journal article
Source: TOXICOLOGY IN VITRO; v. 24, n. 7, p. 1911-1919, OCT 2010.
Web of Science Citations: 9
Abstract

Photoderivatives of riboflavin can modulate the proliferation and survival of cancer cells. In this work, we examined the influence of riboflavin and photoderivatives on osteoblast differentiation induced by ascorbic acid and beta-glycerophosphate. These compounds decreased the osteoblast proliferation, increased the alkaline phosphatase activity, promoted a reduction in matrix metalloproteinase-2 activity and the decreased in the OPG/RANKL ratio. The effects of flavins on osteoblasts were unrelated to the antioxidant activity of these compounds. The biological activity of osteogenic medium containing riboflavin and its photoderivatives involved the activation of different signaling pathways (AKT, FAK, CaMKII), caspases-3, -8 and -9, and up-regulation of the expression and/or stabilization of osteoblastic transcription factors (Runx2 and beta-catenin). These findings suggest a potential use of flavins as adjuvants to improve bone metabolism. (C) 2010 Elsevier Ltd. All rights reserved. (AU)