| Full text | |
| Author(s): |
da Cunha Tirapelli, Daniela Pretti
[1]
;
Carlotti Junior, Carlos Gilberto
;
Leite, Joao Pereira
[2]
;
Tirapelli, Luis Fernando
;
Colli, Benedicto Oscar
Total Authors: 5
|
| Affiliation: | [1] USP, FMRP, Dept Cirurgia & Anat, Med Sch Ribeirao Preto, BR-14048900 Ribeirao Preto, SP - Brazil
[2] USP, FMRP, Dept Neurosci & Behav Sci, Med Sch Ribeirao Preto, BR-14048900 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Arquivos de Neuro-Psiquiatria; v. 68, n. 4, p. 592-596, AUG 2010. |
| Web of Science Citations: | 7 |
| Abstract | |
Heat shock proteins (HSPs) are molecular chaperones that bind to other proteins to shepherd them across membranes and direct them to specific locations within a cell. Several injurious stimuli can induce Hsp70 expression, including ischemia. This study aimed to investigate the pattern of expression of protein (immunohistochemistry) and gene (real-time PCR) Hsp70 in experimental focal cerebral ischemia in rats by occlusion of the middle cerebral artery for 1 hour and the role of neuroprotection with hypothermia (H) and ketoprofen (K). The infarct volume was measured using morphometric analysis defined by triphenyl tetrazolium chloride. It was observed increases in the protein (p=0.0001) and gene (p=0.0001) Hsp70 receptor in the ischemic areas that were reduced by H (protein and gene: p<0.05), K (protein: p<0.001), and H+K (protein: p<0.01 and gene: p<0.05). The Hsp70 increases in the ischemic area suggests that the Hsp70-mediated neuroexcitotoxicity plays an important role in cell death and that the neuroprotective effect of both, H and K are directly involved with the Hsp70. (AU) | |
| FAPESP's process: | 05/00381-8 - Gene expression apoptosis after focal brain ischemia in rats and neutoprotective effect of hypotermia and ketoprofen |
| Grantee: | Benedicto Oscar Colli |
| Support Opportunities: | Regular Research Grants |