| Full text | |
| Author(s): Show less - |
Caserta, R.
[1, 2]
;
Takita, M. A.
[1]
;
Targon, M. L.
[1]
;
Rosselli-Murai, L. K.
[2]
;
de Souza, A. P.
[2]
;
Peroni, L.
[3]
;
Stach-Machado, D. R.
[3]
;
Andrade, A.
[4]
;
Labate, C. A.
[4]
;
Kitajima, E. W.
[5]
;
Machado, M. A.
[1]
;
de Souza, A. A.
[1]
Total Authors: 12
|
| Affiliation: | [1] Ctr APTA Citros Sylvio Moreira IAC, BR-13490970 Cordeiropolis, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP, Ctr Biol Mol & Engn Genet, Dept Genet & Evolucao, Inst Biol, BR-13083970 Campinas, SP - Brazil
[3] Univ Estadual Campinas, UNICAMP, Lab Imunol Aplicada, Dept Microbiol & Imunol, BR-13083970 Campinas, SP - Brazil
[4] USP, Escola Super Agr Luiz de Queiroz, Lab Max Feffer Genet Plantas, Dept Genet, BR-13400970 Piracicaba, SP - Brazil
[5] USP, Escola Super Agr Luiz de Queiroz, Nucleo Apoio Pesquisa Microscopia Eletron Aplicad, BR-13418900 Piracicaba, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Applied and Environmental Microbiology; v. 76, n. 13, p. 4250-4259, JUL 2010. |
| Web of Science Citations: | 31 |
| Abstract | |
Complete sequencing of the Xylella fastidiosa genome revealed characteristics that have not been described previously for a phytopathogen. One characteristic of this genome was the abundance of genes encoding proteins with adhesion functions related to biofilm formation, an essential step for colonization of a plant host or an insect vector. We examined four of the proteins belonging to this class encoded by genes in the genome of X. fastidiosa: the PilA2 and PilC fimbrial proteins, which are components of the type IV pili, and XadA1 and XadA2, which are afimbrial adhesins. Polyclonal antibodies were raised against these four proteins, and their behavior during biofilm development was assessed by Western blotting and immunofluorescence assays. In addition, immunogold electron microscopy was used to detect these proteins in bacteria present in xylem vessels of three different hosts (citrus, periwinkle, and hibiscus). We verified that these proteins are present in X. fastidiosa biofilms but have differential regulation since the amounts varied temporally during biofilm formation, as well as spatially within the biofilms. The proteins were also detected in bacteria colonizing the xylem vessels of infected plants. (AU) | |
| FAPESP's process: | 08/57909-2 - Genomic platforms applied to citrus breeding |
| Grantee: | Marcos Antonio Machado |
| Support Opportunities: | Research Projects - Thematic Grants |