| Full text | |
| Author(s): |
Martinelli, Arturo B.
[1]
;
De Moraes-Barros, Nadia
[2]
;
Alvarenga, Clara S.
[1]
;
Chaves, Paulo B.
[1]
;
Santos, Luiz A. D.
[1]
;
Fagundes, Valeria
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Fed Espirito Santo, LGA, Dept Ciencias Biol, Ctr Ciencias Humanas & Nat, BR-29075910 Vitoria, ES - Brazil
[2] Univ Sao Paulo, Lab Biol Evolut & Conservacao Vertebrados Labec, Dept Genet & Biol Evolut, Inst Biociencias, BR-05508090 Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | MOLECULAR ECOLOGY RESOURCES; v. 10, n. 4, p. 732-734, JUL 2010. |
| Web of Science Citations: | 1 |
| Abstract | |
The three-toed sloths (Bradypus) are slow-moving arboreal neotropical mammals. Understanding demographic variables (such as sex ratio) of populations is a key for conservation purposes. Nevertheless, gender assignment of Bradypus is particularly challenging because of the lack of sexual dimorphism in infants and in adults, particularly B. torquatus, the most endangered of the three-toed sloths, in which sex is attributed by visual observation of the reproductively active males. Here, we standardized a method for sexing Bradypus individuals using PCR-RFLP of sex-linked genes ZFX/ZFY. This assay was validated with known-gender animals and proved accurate to assign gender on three Bradypus species. (AU) | |