| Full text | |
| Author(s): |
Gimenez, Marcela
[1, 2]
;
de Oliveira Souza, Vanessa Cristina
[1, 2]
;
Izumi, Clarice
[1]
;
Barbieri, Manuela R.
[1, 2]
;
Chammas, Roger
[2, 3]
;
Oba-Shinjo, Sueli Mieko
[4]
;
Uno, Miyuki
[4]
;
Nagahashi Marie, Suely Kazue
[4]
;
Rosa, Jose Cesar
[1, 2]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Mol & Cell Biol, Sao Paulo - Brazil
[2] Fundacao Hemoctr Ribeirao Preto, CEPID CTC Ctr Cell Therapy, Ribeirao Preto - Brazil
[3] Lab Expt Oncol LIM 24, Dept Radiol, Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Med Sao Paulo, Dept Neurol, Mol & Cellular Biol Lab, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | PROTEOMICS; v. 10, n. 15, p. 2812-2821, JUL 2010. |
| Web of Science Citations: | 27 |
| Abstract | |
Proteomic approaches have been useful for the identification of aberrantly expressed proteins in complex diseases such as cancer. These proteins are not only potential disease biomarkers, but also targets for therapy. The aim of this study was to identify differentially expressed proteins in diffuse astrocytoma grade II, anaplastic astrocytoma grade III and glioblastoma multiforme grade IV in human tumor samples and in non-neoplastic brain tissue as control using 2-DE and MS. Tumor and control brain tissue dissection was guided by histological hematoxylin/eosin tissue sections to provide more than 90% of tumor cells and astrocytes. Six proteins were detected as up-regulated in higher grade astrocytomas and the most important finding was nucleophosmin (NPM) (p < 0.05), whereas four proteins were down-regulated, among them raf kinase inhibitor protein (RKIP) (p < 0.05). We report here for the first time the alteration of NPM and RKIP expression in brain cancer. Our focus on these proteins was due to the fact that they are involved in the PI3K/AKT/mTOR and RAS/RAF/MAPK pathways, known for their contribution to the development and progression of gliomas. The proteomic data for NPM and RKIP were confirmed by Western blot, quantitative real-time PCR and immunohistochemistry. Due to the participation of NPM and RKIP in uncontrolled proliferation and evasion of apoptosis, these proteins are likely targets for drug development. (AU) | |
| FAPESP's process: | 04/12133-6 - Search for molecular markers related to the diagnosis and prognosis of tumors of the central nervous system |
| Grantee: | Suely Kazue Nagahashi Marie |
| Support Opportunities: | Research Projects - Thematic Grants |