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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Intake of trans Fatty Acids Causes Nonalcoholic Steatohepatitis and Reduces Adipose Tissue Fat Content

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Author(s):
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Machado, Roberta M. [1] ; Stetano, Jose T. [2] ; Oliveira, Claudia P. M. S. [2] ; Mello, Evandro S. [3] ; Ferreira, Fabiana D. [1] ; Nunes, Valeria S. [1] ; de Lima, Vicencia M. R. [2] ; Quintao, Eder C. R. [1] ; Catanozi, Sergio [1] ; Nakandakare, Edna R. [1] ; Lottenberg, Ana Maria P. [1]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Fac Med Sci Univ, Endocrinol & Metab Div, Lipids Lab, LIM 10, BR-01246903 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Gastroenterol, LIM 07, BR-01246903 Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med Sci, Dept Pathol, LIM 14, BR-01246903 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Nutrition; v. 140, n. 6, p. 1127-1132, JUN 2010.
Web of Science Citations: 45
Abstract

We investigated the effects of dietary trans fatty acids, PUFA, and SEA on body and liver fat content, liver histology, and mRNA of enzymes involved in fatty acid metabolism. LDL receptor knockout weaning male mice were fed for 16 wk with diets containing 40% energy as either trans fatty acids (TRANS), PUFA, or SEA. Afterwards, subcutaneous and epididymal fat were weighed and histological markers of nonalcoholic fatty liver disease (NAFLD) were assessed according to the Histological Scoring System for NAFLD. PPAR alpha, PPAR gamma, microsomal triglyceride transfer protein (MTP), carnitine palmitoyl transferase 1 (CPT-1), and sterol regulatory element binding protein-1c (SREBP-1c) mRNA were measured by quantitative RT-PCR. Food intake was similar in the 3 groups, although mice fed the TRANS diet gained less weight than those receiving the PUFA diet. Compared with the PUFA- and SEA-fed mice, TRANS-fed mice had greater plasma total cholesterol (TC) and triglyceride (TG) concentrations, less epididymal and subcutaneous fat, larger livers with nonalcoholic steatohepatitis (NASH)-like lesions, and greater liver TC and TG concentrations. Macrosteatosis in TRANS-fed mice was associated with a higher homeostasis model assessment of insulin resistance (HOMA(IR)) index and upregulated mRNA related to hepatic fatty acid synthesis (SREBP-1 c and PPAR gamma) and to downregulated MTP mRNA. Diet consumption did not alter hepatic mRNA related to fatty acid oxidation (PPAR alpha and CPT-1). In conclusion, compared with PUFA- and SFA-fed mice, TRANS-fed mice had less adiposity, impaired glucose tolerance characterized by greater HOMA(IR) index, and NASH-like lesions due to greater hepatic lipogenesis. These results demonstrate the role of trans fatty acid intake on the development of key features of metabolic syndrome. J. Nutr. 140: 1127-1132, 2010. (AU)