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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of DNA damage by the alkaline comet assay of the olfactory and respiratory epithelia of dogs from the city of Sao Paulo, Brazil

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Author(s):
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Kimura, K. C. [1] ; Fukumasu, H. [1] ; Chaible, L. M. [1] ; Lima, C. E. [1] ; Horst, M. A. [1] ; Matsuzaki, P. [1] ; Sanches, D. S. [1] ; Pires, C. G. [1] ; Silva, T. C. [1] ; Pereira, T. C. [1] ; Mello, M. L. [2] ; Matera, J. M. [3] ; Dias, R. A. [4] ; Monnereau, A. [5, 6] ; Sasco, A. J. [6] ; Saldiva, P. H. N. [7] ; Dagli, M. L. Z. [1]
Total Authors: 17
Affiliation:
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, BR-05508900 Sao Paulo - Brazil
[2] Univ Estadual Campinas, Dept Cell Biol, Campinas - Brazil
[3] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Surg, BR-05508900 Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Prevent Vet Med & Anim Hlth, BR-05508900 Sao Paulo - Brazil
[5] Ctr Comprehens Canc, Inst Bergonie, Bordeaux - France
[6] Victor Segalen Bordeaux 2 Univ, INSERM, U897, Bordeaux - France
[7] Univ Sao Paulo, Sch Med, Dept Pathol, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 7
Document type: Journal article
Source: EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY; v. 62, n. 3, p. 209-219, MAY 2010.
Web of Science Citations: 4
Abstract

Animals kept as pets may be considered sentinels for environmental factors to which humans could be exposed. Olfactory and respiratory epithelia are directly subjected to airborne factors, which could cause DNA lesions, and the alkaline comet assay is considered a reliable tool for the assessment of DNA damage. The objective of this work is to evaluate the extent of DNA damage by the comet assay of the olfactory and respiratory epithelia of dogs from different regions of the city of sao Paulo, Brazil. Thirty-three clinically healthy dogs, aged 5 years or more, were used in the study, with 7 from the North region of Sao Paulo, 7 from the South region, 3 dogs from the East region, and 16 dogs from the West city region. Three dogs younger than 6 months were used as controls. DNA damage was analyzed by the alkaline comet assay. We observed no difference in histopathological analysis of olfactory and respiratory epithelia between dogs from different regions of Sao Paulo. Dogs older than 5 years presented significantly higher comet length in both olfactory and respiratory epithelia, when compared with controls, indicating DNA damage. When separated by regions, olfactory and respiratory epithelia presented similar DNA damage in dogs from different regions of Sao Paulo, corroborating with similar levels of particulate matter index (PM10) in all regions of the city. In this study, we report for the first time that the comet assay can be used to quantify the extent of DNA damage in dog olfactory and respiratory epithelia, and that comet length (DNA damage) increases with age, probably due to environmental factors. Air pollution, as measured by PM 10, can be responsible for this DNA damage. (C) 2009 Elsevier GmbH. All rights reserved. (AU)