Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cloning and endogenous expression of a Eucalyptus grandis UDP-glucose dehydrogenase cDNA

Full text
Author(s):
Show less -
Veneziano Labate, Monica T. [1] ; Ferreira Bertolo, Ana L. [1] ; do Nascimento, Daniela Defavari [1] ; Gutmanis, Gunta [1] ; de Andrade, Alexander [1] ; Calderan Rodrigues, Maria J. [1] ; Camargo, Eduardo L. O. [1] ; Boaretto, Luis Felipe [1] ; Moon, David H. [1] ; Bragatto, Juliano [1] ; Labate, Carlos A. [1]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, Lab Max Feffer Genet Plantas, BR-13418900 Piracicaba, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: GENETICS AND MOLECULAR BIOLOGY; v. 33, n. 4, p. 686-U131, 2010.
Web of Science Citations: 4
Abstract

UDP-glucose dehydrogenase (UGDH) catalyzes the oxidation of UDP-glucose (UDP-Glc) to UDP-glucuronate (UDP-GlcA), a key sugar nucleotide involved in the biosynthesis of plant cell wall polysaccharides. A full-length cDNA fragment coding for UGDH was cloned from the cambial region of 6-month-old E. grandis saplings by RT-PCR. The 1443-bp-ORF encodes a protein of 480 amino acids with a predicted molecular weight of 53 kDa. The recombinant protein expressed in Escherichia coli catalyzed the conversion of UDP-Glc to UDP-GlcA, confirming that the cloned cDNA encodes UGDH. The deduced amino acid sequence of the cDNA showed a high degree of identity with UGDH from several plant species. The Southern blot assay indicated that more than one copy of UGDH is present in Eucalyptus. These results were also confirmed by the proteomic analysis of the cambial region of 3- and 22-year-old E. grandis trees by 2-DE and LC-MS/MS, showing that at least two isoforms are present. The cloned gene is mainly expressed in roots, stem and bark of 6-month-old saplings, with a lower expression in leaves. High expression levels were also observed in the cambial region of 3- and 22-year-old trees. The results described in this paper provide a further view of the hemicellulose biosynthesis during wood formation in E. grandis. (AU)

FAPESP's process: 01/11080-8 - Alteration of the quality of Eucalyptus wood
Grantee:Carlos Alberto Labate
Support Opportunities: Research Grants - Research Partnership for Technological Innovation - PITE