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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prevalence of Mycoplasma genitalium among HIV-infected men in Sao Paulo city detected by realtime polymerase chain reaction

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Author(s):
da Costa, F. A. M. [1, 2] ; da Silva, R. C. [3] ; Arruda, L. B. [4] ; Montanheiro, P. ; da Silva Duarte, A. J. [1, 2] ; Casseb, J. [1, 2]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sch Med, Dept Dermatol, Sao Paulo - Brazil
[2] FMUSP, LIM 56, Lab Allergy & Immunodeficiences, Sao Paulo - Brazil
[3] CRT AIDS, Ctr Reference Treatment HIV AIDS, Sao Paulo - Brazil
[4] Univ Sao Paulo, Inst Trop Med, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF STD & AIDS; v. 21, n. 1, p. 23-25, JAN 2010.
Web of Science Citations: 6
Abstract

Genital mycoplasmas are natural inhabitants of the male urethra and are potentially pathogenic species playing an aetiological role in both genital infections and male infertility. This study aims to determine the presence of Mycoplasma genitalium DNA in urine samples of HIV-1-infected men in Sao Paulo city. Realtime polymerase chain reaction (PCR) was performed using the primers My-ins and Mgso-2 and the Taqman probe Mgen-P1 as described previously. A total of 223 HIV-1-infected men were tested with a mean age of 44 years. Thirteen (5.8%) presented M. genitalium in urine and the co-infection was more common among homosexual men (76.9% versus 51.9%, P < 0.26). In conclusion, realtime PCR was a useful and rapid method for detecting M. genitalium DNA in urine samples. Further studies should be conducted to assess the clinical significance of these results on HIV transmission and its impact on HIV viral load. (AU)