| Full text | |
| Author(s): |
Nascimento, Ivan P.
[1]
;
Dias, Waldely O.
[1]
;
Quintilio, Wagner
[2]
;
Hsu, Tsungda
[3]
;
Jacobs, Jr., William R.
[4, 3]
;
Leite, Luciana C. C.
[1]
Total Authors: 6
|
| Affiliation: | [1] Inst Butantan, Ctr Biotecnol, BR-05503900 Sao Paulo - Brazil
[2] Inst Butantan, Div Controle Qualidade, BR-05503900 Sao Paulo - Brazil
[3] Albert Einstein Coll Med, Howard Hughes Med Inst, Bronx, NY 10461 - USA
[4] Albert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10461 - USA
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Vaccine; v. 27, n. 52, p. 7346-7351, DEC 9 2009. |
| Web of Science Citations: | 17 |
| Abstract | |
Mycobacterium bovis BCG has long been investigated as a candidate for heterologous antigen presentation. We have previously described an rBCG-Pertussis that confers protection against challenge with Bordetella pertussis in neonate and adult mice. In order to obtain stable expression in vivo, we constructed an unmarked BCG lysine auxotrophic and a complementation vector containing the lysine and the genetically detoxified S1 pertussis toxin genes, both under control of the same promoter. Complemented BCG-Delta lysine growth and expression of the pertussis antigen were stable, without the use of an antibiotic marker. Our results show that the complemented rBCG-Delta lysA-S1PT-lysA(+)(kan(-)), which is now suitable to be evaluated in clinical trials, maintains similar characteristics of the original rBCG-pNL71S1PT strain, such as the antigen expression level, cellular immune response and protection against the same model challenge in neonatal-immunized mice. (C) 2009 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 96/11539-0 - Desenvolvimento de uma vacina de bcg recombinante - dpt. |
| Grantee: | Luciana Cezar de Cerqueira Leite |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 02/01748-4 - Influencia das propriedades adjuvantes de toxinas bacterianas expressas em bcg recombinante na resposta induzida contra micobacterias. |
| Grantee: | Ivan Pereira Nascimento |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |