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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Enteroinvasive Escherichia coli vs. Shigella flexneri : how different patterns of gene expression affect virulence

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Author(s):
Ramos Moreno, Ana Carolina [1] ; Ferreira, Lucas Goncalves [1] ; Martinez, Marina Baquerizo [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: FEMS Microbiology Letters; v. 301, n. 2, p. 156-163, DEC 2009.
Web of Science Citations: 17
Abstract

Important features of the enteroinvasive Escherichia coli (EIEC) phenotype and gene expression likely to confer EIEC with a lower ability to cause disease than Shigella flexneri were described here for the first time. To confirm the lower pathogenicity of EIEC, we have analyzed the keratoconjunctivitis developed in guinea-pigs with EIEC or S. flexneri. Shigella flexneri induced a more pronounced proinflammatory response, whereas EIEC induced a mild form of the disease. EIEC showed a significantly less efficient cell-to-cell Caco-2 dissemination when compared with S. flexneri. Plaques formed by EIEC during intercellular spreading were four times smaller than those formed by S. flexneri. At the molecular level, the lower expression of virulence genes by EIEC during infection of Caco-2 cells highlighted the importance of effective gene transcription for bacterial pathogenicity. (AU)