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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Drosophila mediopunctata microsatellites II: cross-species amplification in the tripunctata group and other Drosophila species

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Author(s):
Laborda, Prianda R. [1] ; Klaczko, Louis B. [2] ; de Souza, Anete P. [1, 3]
Total Authors: 3
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, CBMEG, BR-13083875 Campinas, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Genet Evolucao & Bioagentes, BR-13083875 Campinas, SP - Brazil
[3] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biol Vegetal, BR-13083875 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: CONSERVATION GENETICS RESOURCES; v. 1, n. 1, p. 281-296, DEC 2009.
Web of Science Citations: 2
Abstract

Heterologous amplification of 134 recently developed Drosophila mediopunctata microsatellites is here reported. Cross-species amplification was analyzed in 30 species of the Drosophila and Sophophora subgenera, and emphasis was given to the tripunctata group. The overall microsatellite transfer rate was 48%. The highest cross-species amplifications were observed in D. unipunctata (n = 94) and D. roehrae (n = 82). The phylogenetic proximity to D. mediopunctata did not correspond to greater numbers of successfully amplified microsatellites. Of the 134 microsatellite loci, 21 failed to amplify in all species and 18 were detected in all of them. A UPGMA dendrogram, based on Jaccard's similarities in the tripunctata group, recovered few expected phylogenetic relationships. The data demonstrated that many D. mediopunctata microsatellites are now available as potentially useful markers for genetic investigation and conservation of other Drosophila species. (AU)