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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The effects of nicotine and cotinine on Porphyromonas gingivalis colonisation of epithelial cells

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Author(s):
Cogo, Karina [1, 2] ; Calvi, Bruna Maria [2] ; Mariano, Flavia Sammartino [3] ; Nobre Franco, Gilson Cesar [1, 2] ; Goncalves, Reginaldo Bruno [3] ; Groppo, Francisco Carlos [2]
Total Authors: 6
Affiliation:
[1] Univ Taubate, Dept Dent, BR-12020330 Taubate, SP - Brazil
[2] State Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Physiol Sci, Area Pharmacol Anesthesiol & Therapeut, Piracicaba, SP - Brazil
[3] State Univ Campinas UNICAMP, Piracicaba Dent Sch, Area Microbiol & Immunol, Dept Oral Diagnost, Piracicaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ARCHIVES OF ORAL BIOLOGY; v. 54, n. 11, p. 1061-1067, NOV 2009.
Web of Science Citations: 22
Abstract

Smoking is a risk factor for development of periodontitis. Porphyromonas gingivalis is an important colonizer of the subgingival crevice and is a major pathogenic agent in the initiation and progression of severe forms of periodontal disease. However, the effect of major cigarette's derivatives on P. gingivalis is poorly understood. The purpose of this study was to determine the influence of nicotine and cotinine on bacterial colonisation to epithelial cells. KB cells monolayers and P. gingivalis ATCC 33277 were exposed to 0.1, 10 and 100 mu g/ml, of nicotine and cotinine concentrations. The epithelial cells were incubated for 24 h while P. gingivalis was exposed to these substances until reach early logarithmic phase. After the incubation period, P. gingivalis ability to colonize KB cells was assayed. The number of cell-associated/invasive bacteria was assessed by counting the colony-forming unities. 100 mu g/ml. cotinine significantly increased P. gingivalis association and invasion of epithelial cells, when the bacteria was exposed to this substance (p < 0.05; ANOVA - Tukey test). No other condition or drug altered the bacteria colonisation ability (p > 0.05). These data indicated that cotinine may interfere with P. gingivalis ability to associate and invade the epithelial cells. Further studies are needed to investigate whether oral cells might be more susceptible to be colonized by P. gingivalis in smokers. (C) 2009 Elsevier Ltd. All rights reserved. (AU)