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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A method to immunolabel rodent spinal cord neurons and glia for molecular study in specific laser microdissected cells involved in neurodegenerative disorders

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Author(s):
de Oliveira, Gabriela Pintar [1] ; Maximino, Jessica Ruivo [1] ; Lin, Chin Jia [2] ; Chadi, Gerson [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Sch Med, Dept Neurol, Neuroregenerat Ctr, BR-01246903 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Dept Pathol, Lab Mol Pathol, BR-01246903 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Molecular Histology; v. 40, n. 3, p. 217-225, JUN 2009.
Web of Science Citations: 4
Abstract

Neuron-glia interaction is involved in physiological function of neurons, however, recent evidences have suggested glial cells as participants in neurotoxic and neurotrophic mechanisms of neurodegenerative/neuroregenerative processes. Laser microdissection offers a unique opportunity to study molecular regulation in specific immunolabeled cell types. However, an adequate protocol to allow morphological and molecular analysis of rodent spinal cord astrocyte, microglia and motoneurons remains a big challenge. In this paper we present a quick method to immunolabel those cells in flash frozen sections to be used in molecular biology analyses after laser microdissection and pressure catapulting. (AU)