| Full text | |
| Author(s): |
de Paula, Viviane Ferraz
[1]
;
Ribeiro, Alison
[1]
;
Pinheiro, Milena L.
[1]
;
Sakai, Monica
[1]
;
Lacava, Mariana C. R.
[2]
;
Lapachinske, Silvio F.
[2]
;
Moreau, Regina L. M.
[2]
;
Palermo-Neto, Joao
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Sch Vet Med, Dept Pathol, Lab Neuroimmunomodulat, Sao Paulo - Brazil
[2] Univ Sao Paulo, Coll Pharmaceut Sci & Toxicol, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | NEUROIMMUNOMODULATION; v. 16, n. 3, p. 191-200, 2009. |
| Web of Science Citations: | 8 |
| Abstract | |
Objective: Looking for possible neuroimmune relationships, we analyzed the effects of methylenedioxymethamphetamine (MDMA) administration on neuroendocrine, neutrophil activity and leukocyte distribution in mice. Methods: Five experiments were performed. In the first, mice were treated with MDMA (10 mg/kg) 30, 60 min and 24 h prior to blood sample collection for neutrophil activity analysis. In the second experiment, the blood of nave mice was collected and incubated with MDMA for neutrophil activity in vitro analysis. In the third and fourth experiments, mice were injected with MDMA (10 mg/kg) and 60 min later, blood and brain were collected to analyze corticosterone serum levels and hypothalamic noradrenaline (NA) levels and turnover. In the last experiment, mice were injected with MDMA 10 mg/kg and 60 min later, blood, bone marrow and spleen were collected for leukocyte distribution analysis. Results: Results showed an increase in hypothalamic NA turnover and corticosterone serum levels 60 min after MDMA (10 mg/kg) administration, a decrease in peripheral blood neutrophil oxidative burst and a decrease in the percentage and intensity of neutrophil phagocytosis. It was further found that MDMA (10 mg/kg) treatment also altered leukocyte distribution in blood, bone marrow and spleen. In addition, no effects were observed for MDMA after in vitro exposure both in neutrophil oxidative burst and phagocytosis. Conclusion: The effects of MDMA administration (10 mg/kg) on neutrophil activity and leukocyte distribution might have been induced indirectly through noradrenergic neurons and/or hypothalamic-pituitary-adrenal axis activations. Copyright (C) 2009 S. Karger AG, Basel (AU) | |
| FAPESP's process: | 04/14128-0 - Neuroimmunomodulation: effects of drugs, stress and cytocines on central nervous and immune systems bidirectional relationships |
| Grantee: | João Palermo Neto |
| Support Opportunities: | Research Projects - Thematic Grants |