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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Micromorphology of Resin/Dentin Interfaces Using 4th and 5th Generation Dual-curing Adhesive/Cement Systems: A Confocal Laser Scanning Microscope Analysis

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Author(s):
Arrais, Cesar A. G. [1] ; Miyake, Katsuia [2] ; Rueggeberg, Frederick A. [3] ; Pashley, David H. [4] ; Giannini, Marcelo [5]
Total Authors: 5
Affiliation:
[1] Univ Guarulhos, Dept Operat Dent, Sch Dent, Guarulhos, SP - Brazil
[2] Med Coll Georgia, IMMAG, Cell Imaging Core Facil, Augusta, GA 30912 - USA
[3] Med Coll Georgia, Sch Dent, Dent Mat Sect, Dept Oral Rehabil, Augusta, GA 30912 - USA
[4] Med Coll Georgia, Dept Oral Biol & Maxillofacial Pathol, Augusta, GA 30912 - USA
[5] Univ Estadual Campinas, Dept Restorat Dent, Piracicaba Sch Dent, Piracicaba, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Journal of Adhesive Dentistry; v. 11, n. 1, p. 15-26, 2009.
Web of Science Citations: 14
Abstract

Purpose: This study evaluated the differential composition of resin/dentin interfaces of indirect restorations created by the application of 4th and 5th generation dual-curing luting systems (bonding agents/resin cements), when each material was either light cured or allowed to self-cure. Materials and Methods: Occlusal flat dentin surfaces of 60 human third molars were assigned into 12 groups (n = 5) according to curing mode and dual-curing cementing system: 4th generation All Bond2 (AB2)/Duolink (Bisco) and 5th generation (B1) Bond1/Lute-it (Pentron). Fluorescein-labeled dextran (FDx) was mixed with the bonding agents, while rhodamine-labeled dextran (RhDx) was incorporated into resin cements and Pre-Bond resin from AB2. Resin cements were applied to 2-mm-thick, precured resin composite disks (Z250, 3M ESPE), which were fixed to dentin surfaces containing adhesive resin in either cured (light cured; LC) or uncured (self-cured; SC) states. The restored teeth were light activated (XL3000, 3M ESPE) according to the manufacturers' instructions (LRC) or allowed to self-cure (SRC), were stored for 24 h, and then vertically, serially sectioned into 1-mm-thick slabs, which were analyzed using confocal laser scanning microscopy. Fluorescent additives indicated where individual components of the bonding/cement systems were located. Additional specimens were prepared and analyzed using a conventional scanning electron microscope. Results: AB2/LC and B1/LC exhibited nonuniform primer/adhesive layer thickness. AB2/SC showed adhesive resin penetration within the primed dentin, and resin cement penetration at the entrance of the dentin tubules. B1/SC/LRC demonstrated resin cement penetration within the hybrid layer and into the dentin tubules. More resin cement penetration was observed in B1/SC/SRC groups than in its LRC equivalent. Conclusion: The morphological features and component interactions among materials at resin/dentin interfaces are related to the activation modes of the primer/adhesive layer and of the resin cement used. (AU)