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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Plasmodium vivax: Microsatellite analysis of multiple-clone infections

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Author(s):
Havryliuk, Tatiana [1, 2] ; Orjuela-Sanchez, Pamela [1] ; Ferreira, Marcelo U. [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Dept Parasitol, Inst Biomed Sci, Sao Paulo - Brazil
[2] Mt Sinai Sch Med, New York, NY - USA
Total Affiliations: 2
Document type: Journal article
Source: Experimental Parasitology; v. 120, n. 4, p. 330-336, DEC 2008.
Web of Science Citations: 21
Abstract

We used mixtures of genomic DNA from two genetically distinct isolates from Brazil, 42M and 312M, to investigate how accurately 12-locus microsatellite typing describes the overall genetic diversity and characterizes multilocus haplotypes in multiple-clone Plasmodium vivax infections. We found varying PCR amplification efficiencies of microsatellite alleles; for example, from the same 1:1 mixture of 42M and 312M DNA we amplified predominantly 312M-type alleles at 10 loci and 42M-type alleles at 2 loci. All microsatellite alleles were accurately scored in 1:0.5 and 1:0.25 312M:42M DNA mixtures, even when minor peak heights did not meet previously suggested criteria for minor allele detection in multiple-clone infections. Relative proportions of major and minor alleles were unaffected by multiple displacement amplification of template DNA prior to PCR-based microsatellite typing. Although microsatellite typing may detect minor alleles in clone mixtures, amplification biases may lead to inaccurate assignment of predominant haplotypes in multiple-clone P. vivax infections. (C) 2008 Elsevier Inc. All rights reserved. (AU)