| Full text | |
| Author(s): |
Toledo, Karina Alves
[1]
;
Pereira, Fernando Lourenco
[1]
;
Mambole, Agnes
[2]
;
Lesavre, Philippe
[2]
;
Roque-Barreira, Maria Cristina
[1]
;
Halbwachs-Mecarelli, Lise
[2]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, FMRP, Dept Biol Celular Mol, BR-14049900 Ribeirao Preto - Brazil
[2] Univ Paris 05, INSERM, U845, F-75015 Paris - France
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Biochemical and Biophysical Research Communications; v. 376, n. 4, p. 764-769, NOV 28 2008. |
| Web of Science Citations: | 1 |
| Abstract | |
The macro phage-derived neutrophil chemotactic factor (MNCF) is an alpha-galactoside-binding lectin, known to induce dexamethasone-insensitive neutrophil recruitment. We further characterized MNCF effects on neutrophils and showed that it shares with TNF-alpha the ability to delay apoptosis and to trigger degranulation. MNCF and TNF-alpha effects show similar kinetics and involve Src kinases and MAPKinases dependent pathways. They were, however, clearly distinguished, since the soluble TNF-receptor etanercept prevented TNF but not MNCF effects, while melibiose disaccharide inhibited MNCF but not TNF effects. Absorption of MNCF on detoxi-gel did not alter its properties, precluding an LPS contamination effect. By contrast, galectin-3 required LPS to activate neutrophils. Specific antibodies allowed to further demonstrate that MNCF and galectin-3 are two distinct molecules. Finally, MNCF- and IL-8-induced neutrophil activation differed by their kinetic and sensitivity to pertussis toxin. In conclusion, MNCF is a distinct neutrophil agonist, with pro-inflammatory activities involving its carbohydrate recognition domain. (C) 2008 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 06/60642-2 - Lectins: biological effects and pharmaceutical applications |
| Grantee: | Maria Cristina Roque Antunes Barreira |
| Support Opportunities: | Research Projects - Thematic Grants |