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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Structural and phylogenetic relationship of ORF 31 from the Anticarsia gemmatalis MNPV to poly (ADP-ribose) polymerases (PARP)

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Author(s):
de Castro Oliveira, Juliana Velasco [1] ; de Melo, Fernando Lucas [1] ; Romano, Camila Malta [1] ; Iamarino, Atila [1] ; Rizzi, Thais Sampaio [1] ; Yeda, Fernanda Peres [2] ; Harsi, Charlotte Marianna [2] ; Caldas Wolff, Jose Luiz [3] ; de Andrade Zanotto, Paolo Marinho [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Lab Evolucao Mol & Bioinformat, BR-05508 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Lab Biol Mol Adenovirus, BR-05508 Sao Paulo - Brazil
[3] Univ Mogi das Cruzes, Nucleo Integrado Biotecnol, Mol Virol Lab, Mogi Das Cruzes, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: VIRUS GENES; v. 37, n. 2, p. 177-184, OCT 2008.
Web of Science Citations: 1
Abstract

ORF 31 is a unique baculovirus gene in the genome of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D). It encodes a putative polypeptide of 369 aa homologous to poly (ADP-ribose) polymerase (PARP) found in the genomes of several organisms. Moreover, we found a phylogenetic association with Group I PARP proteins and a 3D homology model of its conserved PARP C-terminal catalytic domain indicating that had almost an exact spatial superimposition of < 1 angstrom with other PARP available structures. The 5' end of ORF 31 mRNA was located at the first nucleotide of a CATT motif at position -27. Using real-time PCR we detected transcripts at 3 h post-infection (p.i.) increasing until 24 h p.i., which coincides with the onset of DNA replication, suggestive of a possible role in DNA metabolism. (AU)