Adherence and invasion of Bacteroidales isolated from the human intestinal tract

Author(s):Show less -

Nakano, V. ^[1] ; Piazza, R. M. F. ^[2] ; Cianciarullo, A. M. ; Bueris, V. ^[2] ; Santos, M. F. ^[2] ; Menezes, M. A. ^[2] ; Mendes-Ledesma, M. R. B. ^[2] ; Szulczewski, V. ; Elias, W. P. ^[2] ; Pumbwe, L. ^{[3, 4]} ; Wexler, H. ^{[3, 4]} ; Avila-Campos, M. J. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Lab Anaerobios, Dept Microbiol, BR-05508 Sao Paulo - Brazil

^[2] Inst Butantan, Bacteriol Lab, Genet Lab, Sao Paulo - Brazil

^[3] Univ Calif Los Angeles, Dept Med, Los Angeles, CA 90024 - USA

^[4] Univ Calif Los Angeles, Greater Los Angeles Vet Adm Healthcare, Los Angeles, CA - USA

• Document type: Journal article
• Source: Clinical Microbiology and Infection; v. 14, n. 10, p. 955-963, OCT 2008.
• Web of Science Citations: 6

Abstract

Members of the genera Bacteroides and Parabacteroides are important constituents of both human and animal intestinal microbiota, and are significant facultative pathogens. In this study, the ability of Bacteroides spp. and Parabacteroides distasonis isolated from both diarrhoeal and normal stools (n = 114) to adhere to and invade HEp-2 cells was evaluated. The presence of putative virulence factors such as capsule and fimbriae was also investigated. Adherence to HEp-2 cells was observed in 75.4% of the strains, which displayed non-localized clusters. Invasion was observed in 37.5% and 26% of the strains isolated from diarrhoeal and non-diarrhoeal stools, respectively. All strains displayed a capsule, whereas none of them showed fimbriae-like structures. This is the first report of the ability of Bacteroides spp. and P. distasonis to adhere to and invade cultured HEp-2 epithelial cells. (AU)