| Full text | |
| Author(s): Show less - |
Meira, D. A.
[1]
;
Almeida, R. A. M. B.
[1]
;
Barbosa, A. N.
[1]
;
de Souza, L. R.
[1]
;
Olivo, T. E. T.
[1]
;
Henriques, R. M. S.
[1]
;
Golim, M. A.
[1]
;
Araujo Jr, J. P.
[2]
;
Nagoshi, L. R.
[1]
;
Orikaza, C. M.
[1]
;
Calvi, S. A.
[1]
Total Authors: 11
|
| Affiliation: | [1] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Trop Dis, Botucatu, SP - Brazil
[2] Araujo Jr, Jr., J. P., Sao Paulo State Univ, UNESP, Botucatu Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Journal of Venomous Animals and Toxins including Tropical Diseases; v. 14, n. 4, p. 685-702, 2008. |
| Web of Science Citations: | 1 |
| Abstract | |
A cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G(1) = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G(2) = 27); and patients on HAART with undetectable VL for at least the past six months (G(3) = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G(4) = 20), which was the control group. Serum cytokine levels (values in pg/mL) were measured by enzyme-linked immunosorbent assay (ELISA) and specific mRNA expression by reverse transcription polymerase chain reaction (RT-PCR). Both techniques were performed on the four groups for TNF-alpha, IL-2, INF-gamma, IL-4 and IL-10. All patients were submitted to VL determination and CD4(+) and CD8(+) T lymphocyte counts. The analysis of the results revealed a significant comparison among groups for both methods and an association between the latter (>80% - r(2) > 0.80). There was only one exception, in control individuals for IL-2 by ELISA. The cytokine profiles, in both methods, for the three patient groups, were mature Th-0. The behaviors of IL-2 and INF-gamma required emphasis due to consequent expression of dominant Th profile. Both methods showed low IL-2 and high mean values of INF-gamma in the three groups. Several authors have recently drawn attention to the substantial apoptosis of infected and non-infected CD4(+) T cells, mainly during primary infection, persisting only in those with INF-gamma phenotype producer and not IL-2. HIV infected individuals submitted to HAART are expected to produce IL-2 in an attempt to present Th-1 profile, but in most cases this did not occur. (AU) | |