| Full text | |
| Author(s): |
Guzzo, Cristiane R.
;
Nagem, Ronaldo A. P.
;
Galvão-Botton, Leonor M. P.
;
Guimarães, Beatriz G.
;
Medrano, Francisco J.
;
Barbosa, João A. R. G.
;
Farah, Shaker Chuck
[7]
Total Authors: 7
|
| Document type: | Journal article |
| Source: | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS; v. 61, n. 5, p. 493-495, May 2005. |
| Field of knowledge: | Biological Sciences - Biochemistry |
| Abstract | |
Xanthomonas axonopodis pv. citri YaeQ (XAC2396) is a member of a family of bacterial proteins conserved in several Gram-negative pathogens. Here, the cloning, expression, purification and crystallization of the 182-residue (20.6 kDa) YaeQ protein are described. Recombinant YaeQ containing selenomethionine was crystallized in space group P21 and crystals diffracted to 1.9 Å resolution at a synchrotron source. The unit-cell parameters are a = 39.75, b = 91.88, c = 48.03 Å, β = 108.37°. The calculated Matthews coefficient suggests the presence of two YaeQ molecules in the asymmetric unit. Initial experimental phases were calculated by the multiple-wavelength anomalous dispersion technique and an interpretable electron-density map was obtained. (AU) | |