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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The intrinsic apoptotic signaling pathway in gastric adenocarcinomas of Brazilian patients: Immunoexpression of the Bcl-2 family (Bcl-2, Bcl-x, Bak, Bax, Bad) determined by tissue microarray analysis

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Author(s):
Mesias Barrezueta, Luis Fernando [1] ; Fujiyama Oshima, Celina Tizuko [1] ; Lima, Flavio Oliveira [1] ; Costa, Henrique De Oliveira [1] ; Gomes, Thiago Simao [1] ; Artigiani Neto, Ricardo [1] ; De Ranco, Marcello Fabiano [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo, Dept Pathol, EPM UNIFESP, BR-04023062 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: MOLECULAR MEDICINE REPORTS; v. 3, n. 2, p. 261-267, MAR-APR 2010.
Web of Science Citations: 14
Abstract

This Study was undertaken to investigate the immunoexpression of Bcl-2 family proteins (Bcl-2, Bcl-x, Bax, Bak and Bad) and to evaluate the correlation between the immunoexpression of these proteins and that of cleaved caspase 3, Ki-67 and p53. A tissue microarray (TMA) paraffin block was constructed using gastric carcinoma tissue (test group) and adjacent normal gastric mucosa (control group) from 87 patients who had not previously undergone radiotherapy or chemotherapy. Sections from the TMA block (4 mu m) were subjected to immunohistochemistry for Bcl-2, Bcl-x, Bax, Bak, Bad, p53, Ki-67 and cleaved caspase-3. The slides were evaluated by the semi-quantitative method. and the scores obtained (intensity vs. percentage of staining) were correlated with one another and with the apoptotic index, cellular proliferation and data regarding patient survival. The studied proteins were present in the tumor tissue and in the normal (gastric mucosa, but at different intensities and with differences in the number of positive cells. There was all association between tumor size and p53 expression, and intestinal type adenocarcinoma was positively correlated with the expression of Bax, Bad and Ki-67. The immunoexpression of Bcl-x, Bak, Bad, p53 and Ki-67 showed statistically significant differences between the tumor tissue and the adjacent normal gastric mucosa. There was all association between the expression of Bax, Bak and Bad in the normal gastric mucosa. No correlation between patient survival and the expression of these proteins was observed. Overexpression of the Bcl-x protein in the adenocarcinomas and the difference ill Bcl-x expression between the test and the control group may be related to the anti-apoptotic effect of this protein. The reduced expression of Bak and Bad and the increased expression of p53 and Ki-67 in the adenocarcinomas demonstrate the imbalance between apoptosis and cellular proliferation, which results in uncontrolled tumor cell proliferation. (AU)

FAPESP's process: 04/09932-4 - Apoptosis in tumors
Grantee:Marcello Fabiano de Franco
Support Opportunities: Research Projects - Thematic Grants