Analysis of Single-Nucleotide Polymorphisms in the crt-o and mdr1 Genes of Plasmodium vivax among Chloroquine-Resistant Isolates from the Brazilian Amazon Region

Author(s)

Orjuela-Sanchez, Pamela ^[1] ; de Santana Filho, Franklin Simoes ^[2] ; Machado-Lima, Ariane ^[3] ; Chehuan, Yonne Francis ^[2] ; Farias Costa, Monica Regina ^[2] ; Costa Alecrim, Maria das Gracas ^[2] ; del Portillo, Hernando A. ^{[4, 5]}

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Sao Paulo - Brazil

^[2] Fdn Trop Med Amazonas, Lab Malaria, Manaus, Amazonas - Brazil

^[3] Univ Sao Paulo, Sch Arts Sci & Humanities, Inst Psychiat, Sao Paulo - Brazil

^[4] Inst Catalana Recerca & Estudis Avancats, Barcelona - Spain

^[5] Barcelona Ctr Int Hlth & Res, Barcelona - Spain

• Document type: Journal article
• Source: Antimicrobial Agents and Chemotherapy; v. 53, n. 8, p. 3561-3564, AUG 2009.
• Web of Science Citations: 42

Abstract

Plasmodium vivax parasites with chloroquine resistance (CQR) are already circulating in the Brazilian Amazon. Complete single-nucleotide polymorphism (SNP) analyses of coding and noncoding sequences of the pvmdr1 and pvcrt-o genes revealed no associations with CQR, even if some mutations had not been randomly selected. In addition, striking differences in the topologies and numbers of SNPs in these transporter genes between P. vivax and P. falciparum reinforce the idea that mechanisms other than mutations may explain this virulent phenotype in P. vivax. (AU)

• FAPESP's process: 01/09401-0 - Genomics and post-genomics approaches to study the human malarial parasites Plasmodium vivax and Plasmodium falciparum in the Brazilian Amazon
• Grantee: Hernando Antonio del Portillo Obando
• Support Opportunities: Research Projects - Thematic Grants