Propagation, growth, and carbohydrates of Dendrobium Second Love (Orchidaceae) in vitro as affected by sucrose, light, and dark

Author(s)

Ferreira, Wagner de Melo ^[1] ; Suzuki, Rogerio M. ^[2] ; Pescador, Rosete ^[3] ; Figueiredo-Ribeiro, Rita de Cassia L. ^[4] ; Kerbauy, Gilberto B. ^[5]

Affiliation

^[1] Univ Fed Tocantins, Nucl Estudos Ambientais NEAMB, BR-77500000 Porto Nacl, TO - Brazil

^[2] Inst Bot Sao Paulo, BR-04301902 Sao Paulo - Brazil

^[3] Univ Fed Santa Catarina, Dept Fitotecnia, BR-88034001 Florianopolis, SC - Brazil

^[4] Inst Bot Sao Paulo, Secao Fisiol & Bioquim Plantas, BR-04301902 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Biociencias, Dept Bot, BR-05422970 Sao Paulo - Brazil

• Document type: Journal article
• Source: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT; v. 47, n. 3, p. 420-427, 2011.
• Web of Science Citations: 9

Abstract

In general, plant material grown in vitro has low photosynthetic ability to achieve positive carbon balances. Therefore, a continuous supply of carbohydrates from the culture medium is required, and sucrose has been the most commonly used carbon source. In this paper, we investigate the effects of different sucrose concentrations and the presence and absence of light on the endogenous levels of soluble carbohydrates and starch as well as on the proliferation and growth of Dendrobium Second Love (Orchidaceae) in vitro. The possibility of using etiolated stem segments as a means for micropropagating this hybrid was also verified. The results obtained indicated that the presence and absence of light and the sucrose concentrations used influenced the amounts of soluble carbohydrates and starch and the proliferation of D. Second Love shoots and roots. An increase in sucrose concentration caused a progressive increase in the amounts of total carbohydrates and starch. Under both light conditions, sucrose was the main sugar found in the shoots followed by glucose and fructose. The addition of sucrose to the culture medium up to 2% and 4% was advantageous to the number of shoots produced per explant and the root longitudinal growth in the presence and absence of light, respectively. Shoot and root dry matter and the number of roots formed per explant increased as sucrose concentration was raised up to 6% in both light treatments. The use of dark-grown shoot segments proved to be a useful and reliable alternative for the micropropagation of this hybrid. (AU)

• FAPESP's process: 05/04139-7 - Carbohydrates of tropical species as modulators of ecophysiological processes and as environmental stress response markers
• Grantee: Marcia Regina Braga
• Support Opportunities: Research Projects - Thematic Grants