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(Reference retrieved automatically from Google Scholar through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Determination of doxorubicin in human plasma by excitation-emission matrix fluorescence and multi-way analysis

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Author(s):
Trevisan‚ M.G. ; Poppi‚ R.J.
Total Authors: 2
Document type: Journal article
Source: Analytica Chimica Acta; v. 493, n. 1, p. 69-81, 2003.
Abstract

Direct determination of doxorubicin (DXR), a cytotoxic anthracycline antibiotic, in human plasma was accomplished based on excitation-emission matrix (EEM) fluorescence measurements and multi-way chemometric methods based on parallel factor analysis (PARAFAC) and N-PLS. Several different procedures, such as residual analysis, core consistency diagnostic (CONCORDIA) and split-half analysis were employed to determine the correct number of factors in PARAFAC. These procedures converged to a choice of two factors, attributed to DXR and to the sum of two fluorescence species present in the plasma. Sample PARAFAC loadings were employed to build a regression model against concentration, resulting in a RMSECV of 0.060 mug ml(-1). N-PLS using two factors produced a RMSECV of 0.045 mug ml(-1). Figures of merit (FOM), such as sensitivity (SEN), selectivity (SEL) and limit of detection (LD) were determined for both PARAFAC and N-PLS. (C) 2003 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 99/12124-7 - Exploitation of novel materials and different reaction media for sensitivy/selectivity enhancement in the analyses of biological and environmental samples
Grantee:Elias Ayres Guidetti Zagatto
Support Opportunities: Research Projects - Thematic Grants