Determination of doxorubicin in human plasma by excitation-emission matrix fluorescence and multi-way analysis

Author(s)

Trevisan‚ M.G. ; Poppi‚ R.J.

• Document type: Journal article
• Source: Analytica Chimica Acta; v. 493, n. 1, p. 69-81, 2003.

Abstract

Direct determination of doxorubicin (DXR), a cytotoxic anthracycline antibiotic, in human plasma was accomplished based on excitation-emission matrix (EEM) fluorescence measurements and multi-way chemometric methods based on parallel factor analysis (PARAFAC) and N-PLS. Several different procedures, such as residual analysis, core consistency diagnostic (CONCORDIA) and split-half analysis were employed to determine the correct number of factors in PARAFAC. These procedures converged to a choice of two factors, attributed to DXR and to the sum of two fluorescence species present in the plasma. Sample PARAFAC loadings were employed to build a regression model against concentration, resulting in a RMSECV of 0.060 mug ml(-1). N-PLS using two factors produced a RMSECV of 0.045 mug ml(-1). Figures of merit (FOM), such as sensitivity (SEN), selectivity (SEL) and limit of detection (LD) were determined for both PARAFAC and N-PLS. (C) 2003 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 99/12124-7 - Exploitation of novel materials and different reaction media for sensitivy/selectivity enhancement in the analyses of biological and environmental samples
• Grantee: Elias Ayres Guidetti Zagatto
• Support Opportunities: Research Projects - Thematic Grants