Effect of Bcl-2 overexpression on mitochondrial structure and function

Kowaltowski, Alicia J.; Cosso, Ricardo G.; Campos, Cláudia B.; Fiskum, Gary

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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of Bcl-2 overexpression on mitochondrial structure and function

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Author(s):	Kowaltowski, Alicia J. ^[1] ; Cosso, Ricardo G. ; Campos, Cláudia B. ; Fiskum, Gary Total Authors: 4
Affiliation:	^[1] Universidade de São Paulo (USP). Instituto de Química. Departamento de Bioquímica - Brasil Total Affiliations: 4
Document type:	Journal article
Source:	Journal of Biological Chemistry; v. 277, n. 45, p. 42802-42807, Nov. 2002.
Field of knowledge:	Biological Sciences - Biochemistry
Abstract
Overexpression of the antiapoptotic Bcl-2 protein enhances the uptake of fluorimetric dyes sensitive to mitochondrial membrane potential, suggesting that Bcl-2 changes the mitochondrial proton gradient. In this study, we performed calibrated measurements of mitochondrial respiration, membrane potential, delta-pH, and intramitochondrial [K+] in digitonin-permeabilized PC12 and GT1-7 neural cells that either do not express human Bcl-2 (control transfectants) or that were transfected with and overexpressed the human bcl-2 gene to evaluate whether Bcl-2 alters mitochondrial inner membrane ion transport. (AU)

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