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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of Haemophilus parasuis isolated from Brazilian swine through serotyping, AFLP and PFGE

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Castilla, Karina Salvagni [1] ; Sena de Gobbi, Debora Dirani [1] ; Moreno, Luisa Zanolli [1] ; Paixao, Renata [1] ; Coutinho, Tania Alen [1] ; dos Santos, Jose Lucio [2] ; Moreno, Andrea Micke [1]
Total Authors: 7
[1] Univ Sao Paulo, FMVZ, Lab Sanidade Suina & Virol, BR-05508270 Sao Paulo - Brazil
[2] Microvet, Microbiol Vet Especial, Vicosa, MG - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Research in Veterinary Science; v. 92, n. 3, p. 366-371, JUN 2012.
Web of Science Citations: 18

Haemophilus parasuis infection in pigs is characterized by fibrinous polyserositis, arthritis and meningitis. Despite the fact that traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, molecular-based methods are alternatives for species-specific tests and epidemiological studies. The aim of this study was to characterize H. parasuis field strains from different states of Brazil, employing serotyping and genotyping methods. Serotyping revealed that serovar 4 was the most prevalent (26.1%), followed by serovars 5(17.4%), 14(8.7%), 13 (4.4%) and 2 (4.4%), whereas 39% of the strains were considered as untypeable. AFLP with a single enzyme and PFGE were able to type all isolates tested, generating 34 and 20 different profiles, respectively, including untypeable strains. Besides the slightly higher discrimination index presented by AFLP, PFGE with Not I restriction enzyme showed a better correlation with epidemiological data, grouping strains of the same serovar, animal or farm origin. The results indicated AFLP and PFGE as valuable tools for typing H. parasuis isolates collected in Brazil. (C) 2011 Elsevier Ltd. All rights reserved. (AU)