| Full text | |
| Author(s): |
Ferreira Filho, Antonio Sergio
[1]
;
Quecine, Maria Carolina
[2]
;
Bogas, Andrea Cristina
[2]
;
Rossetto, Priscilla de Barros
[3]
;
de Souza Lima, Andre Oliveira
[4]
;
Lacava, Paulo Teixeira
[5]
;
Azevedo, Joao Lucio
[2]
;
Araujo, Welington Luiz
[6]
Total Authors: 8
|
| Affiliation: | [1] Univ Passo Fundo, Dept Phytopathol, BR-99052900 Passo Fundo, RS - Brazil
[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, BR-13400970 Piracicaba, SP - Brazil
[3] Univ Sao Paulo, Ctr Energia Nucl Agr, Biotechnol Lab, BR-13416000 Piracicaba, SP - Brazil
[4] Univ Vale Itajai, Ctr Sci Tecnol Earh & Sea, BR-88302202 Itajai, SC - Brazil
[5] Univ Fed Alfenas, Inst Nat Sci, BR-37130000 Alfenas, MG - Brazil
[6] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY; v. 28, n. 4, p. 1475-1481, APR 2012. |
| Web of Science Citations: | 10 |
| Abstract | |
Based on the premise of symbiotic control, we genetically modified the citrus endophytic bacterium Methylobacterium extorquens, strain AR1.6/2, and evaluated its capacity to colonize a model plant and its interaction with Xylella fastidiosa, the causative agent of Citrus Variegated Chlorosis (CVC). AR1.6/2 was genetically transformed to express heterologous GFP (Green Fluorescent Protein) and an endoglucanase A (EglA), generating the strains ARGFP and AREglA, respectively. By fluorescence microscopy, it was shown that ARGFP was able to colonize xylem vessels of the Catharanthus roseus seedlings. Using scanning electron microscopy, it was observed that AREglA and X. fastidiosa may co-inhabit the C. roseus vessels. M. extorquens was observed in the xylem with the phytopathogen X. fastidiosa, and appeared to cause a decrease in biofilm formation. AREglA stimulated the production of resistance protein, catalase, in the inoculated plants. This paper reports the successful transformation of AR1.6/2 to generate two different strains with a different gene each, and also indicates that AREglA and X. fastidiosa could interact inside the host plant, suggesting a possible strategy for the symbiotic control of CVC disease. Our results provide an enhanced understanding of the M. extorquens-X. fastidiosa interaction, suggesting the application of AR1.6/2 as an agent of symbiotic control. (AU) | |
| FAPESP's process: | 98/16262-2 - The role of xanthan gum-associated genes in the pathogenicity and virulence of Xylella fastidiosa and potential strategies for biological control of citrus CVC |
| Grantee: | João Lúcio de Azevedo |
| Support Opportunities: | Genome Research Grants |