Identification and characterization of differentially expressed genes during the interaction between Methylobacterium mesophilicum and the host plant

• Grant number: 10/07594-5
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2010
• End date: August 31, 2012
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Welington Luiz de Araújo
• Grantee: Welington Luiz de Araújo
• Host Institution: Pró-Reitoria de Pesquisa, Pós-Graduação e Extensão e Assuntos Comunitários. Universidade de Mogi das Cruzes (UMC). Mogi das Cruzes , SP, Brazil

Abstract

The genus Methylobacterium is composed by Pink Pigmented, Facultative Methylotrophic bacteria (PPFMs), which in some case may colonize endophytically the host plant. Some species of this genus are able to promote plant growth, increase the photosynthetic activity of the host plant and reduce the incidence of pathogens. However, little is known about which genes of Methylobacterium spp. may be involved with the interaction endophyte - host plant. The strain SR1.6 / 6 of M. mesophilicum is a bacterium obtained from citrus that has interaction with Xylella fastidiosa, and has been the subject of many researches about bacteria-plant interaction. Thus, this study aims to sequence the genome of M. mesophilicum and identify genes differentially expressed (transcriptome) during host plant association. Moreover, since other species of Methylobacterium have been sequenced, we will be able to compare the genome of endophytic species (M. nodulans and M. mesophilicum) and non-endophytic (M. chloromethanicum, M. populi and M. radiotolerans). Finally, this study aims to contribute to a better understanding of the interaction endophyte - plant host using the description and quantification of potential differentially expressed genes related to interaction and also, elucidate the strategies used by bacteria for colonization of the plant. This understanding will help in future studies using this interaction in agricultural production, contributing to increased resistance to pathogens and increased plant growth. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MANUELLA NÓBREGA DOURADO; FERNANDO DINI ANDREOTE; FRANCISCO DINI-ANDREOTE; RAPHAEL CONTI; JANETE MAGALI ARAÚJO; WELINGTON LUIZ ARAÚJO. Analysis of 16S rRNA and mxaF genes reveling insights into Methylobacterium niche-specific plant association. GENETICS AND MOLECULAR BIOLOGY, v. 35, n. 1, p. 142-148, 2012-02-03. (10/07594-5)

DOURADO, MANUELLA NOBREGA; SANTOS, DAIENE SOUZA; NUNES, LUIZ ROBERTO; BATISTA DA COSTA DE OLIVEIRA, REGINA LUCIA; DE OLIVEIRA, MARCUS VINICIUS; ARAUJO, WELINGTON LUIZ. Differential gene expression in Xylella fastidiosa 9a5c during co-cultivation with the endophytic bacterium Methylobacterium mesophilicum SR1.6/6. JOURNAL OF BASIC MICROBIOLOGY, v. 55, n. 12, p. 1357-1366, DEC 2015. (12/24217-6, 10/07594-5)

DOURADO, MANUELLA NOBREGA; CAMARGO NEVES, ALINE APARECIDA; SANTOS, DAIENE SOUZA; ARAUJO, WELINGTON LUIZ. Biotechnological and Agronomic Potential of Endophytic Pink-Pigmented Methylotrophic Methylobacterium spp.. BIOMED RESEARCH INTERNATIONAL, v. 2015, p. 19-pg., 2015-01-01. (10/07594-5, 12/24217-6)

DOURADO, MANUELLA NOBREGA; CAMARGO NEVES, ALINE APARECIDA; SANTOS, DAIENE SOUZA; ARAUJO, WELINGTON LUIZ. Biotechnological and Agronomic Potential of Endophytic Pink-Pigmented Methylotrophic Methylobacterium spp.. BIOMED RESEARCH INTERNATIONAL, 2015. (12/24217-6, 10/07594-5)

DOURADO, MANUELLA NOBREGA; BOGAS, ANDREA CRISTINA; POMINI, ARMANDO M.; ANDREOTE, FERNANDO DINI; QUECINE, MARIA CAROLINA; MARSAIOLI, ANITA J.; ARAUJO, WELINGTON LUIZ. Methylobacterium-plant interaction genes regulated by plant exudate and quorum sensing molecules. Brazilian Journal of Microbiology, v. 44, n. 4, p. 1331-1339, OCT-DEC 2013. (10/07594-5)

DINI-ANDREOTE, FRANCISCO; ANDREOTE, FERNANDO DINI; ARAUJO, WELINGTON LUIZ; TREVORS, JACK T.; VAN ELSAS, JAN DIRK. Bacterial Genomes: Habitat Specificity and Uncharted Organisms. MICROBIAL ECOLOGY, v. 64, n. 1, p. 1-7, JUL 2012. (10/07594-5)