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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular characterization of group A bovine rotavirus in southeastern and central-western Brazil, 2009-2010

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Author(s):
Silva, Fernanda D. F. [1] ; Gregori, Fabio [2] ; Goncalves, Ana C. S. [1] ; Samara, Samir Issa [1] ; Buzinaro, Maria G. [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, BR-14884900 Jaboticabal, SP - Brazil
[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Pesquisa Veterinária Brasileira; v. 32, n. 3, p. 237-242, MAR 2012.
Web of Science Citations: 9
Abstract

Rotavirus is an important cause of neonatal diarrhea in humans and several animal species, including calves. A study was conducted to examine 792 fecal samples collected from calves among 65 dairy and beef herds distributed in two of Brazil's major livestock producing regions, aiming to detect the occurrence of rotavirus and perform a molecular characterization of the rotavirus according to G and P genotypes in these regions. A total of 40 (5.05%) samples tested positive for rotavirus by the polyacrylamide gel electrophoresis (PAGE) technique. The molecular characterization was performed by multiplex semi-nested RT-PCR reactions, which indicated that the associations of genotypes circulating in herds in Brazil's southeastern region were G6P[11], G10P[11], G[-]P[5] + [11], G[-]P[6] in the state of São Paulo and G6P[11], G8P[5], G11P[11], G10P[11] in the state of Minas Gerais. In the central-western region, the genotypes G6P[5] + [11], G6P[5], G8P[-], G6P[11], G [-] P[1], G[-] P[11], and G[-] P[5] were detected in the state of Goiás, while the genotypes G6P[5], G8[P11], G6[P11], G8[P1], G8[P5], G6[P1] were circulating in herds in the state of Mato Grosso do Sul. The genotypic diversity of bovine rotavirus found in each region under study underlines the importance of characterizing the circulating samples in order to devise the most effective prophylactic measures. (AU)

FAPESP's process: 10/02997-4 - Occurrence and molecular biodiversity of rotavirus in dairy and beef herds cattle in Brazilian Southeast and Central-West regions.
Grantee:Fernanda Dornelas Florentino Silva
Support Opportunities: Scholarships in Brazil - Master