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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of Immune Cells by Flow Cytometry in Vaginal Lavages from Women with Vulvovaginitis and Normal Microflora

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Author(s):
Giraldo, Paulo Cesar [1, 2] ; Junqueira de Carvalho, Joziani Beghini [1, 2] ; Gomes do Amaral, Rose Luce [1] ; da Silveira Goncalves, Ana Katherine [3] ; Eleuterio, Jr., Jose [4] ; Guimaraes, Fernando [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, Hosp Mulher Prof Dr Jose Aristodemo Pinotti, Ctr Atencao Integral Saude Mulher, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Fac Ciencias Med, Dept Tocoginecol, Campinas, SP - Brazil
[3] Univ Fed Rio Grande do Norte, Fac Med, Dept Obstet & Ginecol, BR-59072970 Natal, RN - Brazil
[4] Univ Fed Ceara, Fac Med, Dept Saude Materno Infantil, Fortaleza, Ceara - Brazil
Total Affiliations: 4
Document type: Journal article
Source: American Journal of Reproductive Immunology; v. 67, n. 3, p. 198-205, MAR 2012.
Web of Science Citations: 14
Abstract

Problem The extent of the vaginal immune response is not fully determined. The purpose of this study was to evaluate the vaginal immune cells from women with vulvovaginitis (VV). Method of Study A total of 142 volunteers diagnosed with bacterial vaginosis (BV), vulvovaginal candidiasis (VC), and BV associated with VC or normal microflora were sampled to evaluate the immune cells by flow cytometry. The immune cells were obtained by vaginal lavage and labeled with fluorochrome- conjugated monoclonal antibodies to identify neutrophil granulocytes, macrophages, CD4 + and CD8 + T lymphocytes, B lymphocytes, and NK lymphocytes. Results Neutrophil granulocytes were present in 84.6% of samples among the leukocyte populations. Considering samples in which neutrophils were present, the mean percentage of neutrophil granulocytes was significantly higher in women with VC than BV and normal microflora and was significantly lower in women with BV than normal microflora. Macrophages and lymphocytes were present in a lower percentage of samples. The mean percentage of CD4 + T lymphocytes in vaginal lavages was significantly higher in VC and BV compared with women with normal microflora. Conclusions Neutrophils were the predominant leukocytes and were associated with VC and inversely with BV. CD4 + T lymphocytes were associated with both VC and BV. (AU)

FAPESP's process: 08/09853-8 - Leucocyte shedding to the vaginal content in women with and without vulvovaginitis
Grantee:Paulo César Giraldo
Support Opportunities: Regular Research Grants