Cytogenetic instability of dental pulp stem cell lines

Author(s)

Duailibi, Monica Talarico ^{[1, 2]} ; Kulikowski, Leslie Domenici ^[3] ; Duailibi, Silvio Eduardo ^{[1, 2, 4]} ; Nunes Lipay, Monica Vannucci ^[5] ; Melaragno, Maria Isabel ; Ferreira, Lydia Masako ^[1] ; Vacanti, Joseph Phillip ^{[6, 7, 8]} ; Yelick, Pamela Crotty ^[9]

Affiliation

^[1] Univ Fed Sao Paulo, Dept Plast Surg, Ctr Cellular & Mol Therapy, UNIFESP CTCMol, Sao Paulo - Brazil

^[2] INCT Biofabris, Natl Inst Sci & Technol, Biofabricat Inst, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Pathol, Cytogenom Lab, BR-05403000 Sao Paulo - Brazil

^[4] UNIFESP Sao Jose Campos, Sci & Technol Inst Biomed Engn, Sao Paulo - Brazil

^[5] Univ Fed Sao Paulo, Div Endocrinol, Sao Paulo - Brazil

^[6] Harvard Univ, Sch Med, Boston, MA - USA

^[7] Massachusetts Gen Hosp, Lab Tissue Engn & Organ Fabricat, Boston, MA 02114 - USA

^[8] Dept Surg, Boston, MA - USA

^[9] Tufts Univ, Dept Oral & Maxillofacial Pathol, Boston, MA 02111 - USA

• Document type: Journal article
• Source: Journal of Molecular Histology; v. 43, n. 1, p. 89-94, FEB 2012.
• Web of Science Citations: 9

Abstract

Human adult stem cells (hASCs) offer a potentially renewable source of cell types that are easily isolated and rapidly expanded for use in regenerative medicine and cell therapies without the complicating ethical problems that are associated with embryonic stem cells. However, the eventual therapeutic use of hASCs requires that these cells and their derivatives maintain their genomic stability. There is currently a lack of systematic studies that are aimed at characterising aberrant chromosomal changes in cultured ASCs over time. However, the presence of mosaicism and accumulation of karyotypic abnormalities within cultured cell subpopulations have been reported. To investigate cytogenetic integrity of cultured human dental stem cell (hDSC) lines, we analysed four expanded hDSC cultures using classical G banding and fluorescent in situ hybridisation (FISH) with X chromosome specific probe. Our preliminary results revealed that about 70% of the cells exhibited karyotypic abnormalities including polyploidy, aneuploidy and ring chromosomes. The heterogeneous spectrum of abnormalities indicates a high frequency of chromosomal mutations that continuously arise upon extended culture. These findings emphasise the need for the careful analysis of the cytogenetic stability of cultured hDSCs before they can be used in clinical therapies. (AU)