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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of varicocele on sperm function and semen oxidative stress

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Author(s):
Blumer, Camile Garcia [1] ; Restelli, Adriana Ester [1] ; Del Giudice, Paula Toni [1] ; Soler, Thiesa Butterby [1] ; Fraietta, Renato [1] ; Nichi, Marcilio [2] ; Bertolla, Ricardo Pimenta [1] ; Cedenho, Agnaldo Pereira [1]
Total Authors: 8
Affiliation:
[1] Univ Fed Sao Paulo, Dept Surg, Div Urol, Human Reprod Sect, BR-04039060 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Anim Reprod, Sch Vet Med, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BJU INTERNATIONAL; v. 109, n. 2, p. 259-265, JAN 2012.
Web of Science Citations: 53
Abstract

OBJECTIVE To assess the effect of varicocele on sperm DNA integrity, mitochondrial activity, lipid peroxidation and acrosome integrity. PATIENTS AND METHODS In all, 30 patients with a clinically diagnosed varicocele of grade II or III and 32 men without a varicocele were evaluated for sperm DNA fragmentation (comet assay), mitochondrial activity (3,3'-diaminobenzidine assay), lipid peroxidation (malondialdehyde) and acrosome integrity (fluorescent probe labelled peanut agglutinin). RESULTS The varicocele group showed fewer spermatozoa with intact DNA (grade II, P = 0.040), more cells with inactive mitochondria (class III, P = 0.001), fewer cells with active mitochondria (class I, P = 0.005) and fewer spermatozoa with intact acrosomes (P < 0.001). Finally, no significant differences were observed in lipid peroxidation levels. CONCLUSION Men with varicocele showed an increase in sperm DNA fragmentation and a reduction in mitochondrial activity and acrosome integrity. However, lipid peroxidation levels remained unchanged. (AU)