Effect of root age on the allocation of metals, amino acids and sugars in different cell fractions of the perennial grass Paspalum notatum (bahiagrass)

Author(s)

Nabais, Cristina ^[1] ; Labuto, Georgia ^[2] ; Goncalves, Susana C. ^[1] ; Buscardo, Erika ^[1] ; Semensatto, Decio ^[2] ; Nogueira, Ana Rita A. ^[3] ; Freitas, Helena ^[1]

Affiliation

^[1] Univ Coimbra, Fac Ciencias & Tecnol, Dept Ciencias Vida, Ctr Ecol Func, P-3001401 Coimbra - Portugal

^[2] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, BR-09972270 Diadema, SP - Brazil

^[3] Embrapa Pecuaria Sudeste, Grp Anal Instrumental Aplicada, BR-13560970 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Plant Physiology and Biochemistry; v. 49, n. 12, p. 1442-1447, DEC 2011.
• Web of Science Citations: 3

Abstract

This work aimed to compare the allocation of Al, Fe, Cu, Ni, amino acids and sugars in different fractions of root cells of Paspalum notatum with 21 and 120 days old grown in quartz sand. In general younger roots showed a higher content of Al, Fe, Cu Ni, amino acids and sugars, compared to older roots. This can be due to a higher metabolic activity of younger roots and/or to structural changes that can occur with cell ageing. Al and Fe were mainly allocated to fractions with pectin, hemicellulose and cellulose, both in younger and older roots. However, older roots also showed a significant fraction of Al allocated to the intracellular fraction. It seems that older roots were less able to prevent the entry of Al in the cytoplasm. The proportion of Cu was higher in intracellular components, both in younger and older roots, as expected from an essential nutrient. Ni content was very low in older roots and in younger roots it was mainly allocated to the intracellular fraction and to the cell wall polysaccharides fraction. The amino acids were mainly allocated to the cytoplasm and polysaccharide fraction. Although younger roots showed a higher total amount of amino acids compared to older roots, the amino acids profile and allocation, mainly in the cytoplasm polysaccharides, was quite similar. Arabinose, a major component of structural glycoproteins of the primary cell wall matrix, was only detected in younger roots. Thus, it seems that root cells of P notatum suffered changes in the composition of the cell wall components with ageing. To further understand the structural changes of root cells with ageing and its effect on metal allocation, it is important to quantify several components of the cell wall matrix, namely pectins and glycoproteins. (C) 2011 Elsevier Masson SAS. All rights reserved. (AU)