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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

CaPrx, a Coffea arabica gene encoding a putative class III peroxidase induced by root-knot nematode infection

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Author(s):
Severino, Fabio E. [1] ; Brandalise, Marcos [1, 2] ; Costa, Carolina S. [1] ; Wilcken, Silvia R. S. [3] ; Maluf, Mirian P. [2, 4] ; Goncalves, Wallace [4] ; Maia, Ivan G. [1]
Total Authors: 7
Affiliation:
[1] UNESP, Inst Biociencias, Dept Genet, BR-18618970 Botucatu, SP - Brazil
[2] EMBRAPA Cafe, Brasilia, DF - Brazil
[3] UNESP, Fac Ciencias Agron, Dept Defesa Fitossanitaria, Botucatu, SP - Brazil
[4] Ctr Cafe Alcides Carvalho, Inst Agron Campinas, Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Plant Science; v. 191, p. 35-42, AUG 2012.
Web of Science Citations: 10
Abstract

Class III peroxidases (Prxs) are enzymes involved in a multitude of physiological and stress-related processes in plants. Here, we report on the characterization of a putative peroxidase-encoding gene from Coffea arabica (CaPrx) that is expressed in early stages of root-knot nematode (RKN) infection. CaPrx showed enhanced expression in coffee roots inoculated with RKN (at 12 h post-inoculation), but no significant difference in expression was observed between susceptible and resistant plants. Assays using transgenic tobacco plants harboring a promoter-beta-glucuronidase (GUS) fusion revealed that the CaPrx promoter was exclusively active in the galls induced by RKN. In cross sections of galls, GUS staining was predominantly localized in giant cells. Up-regulation of GUS expression in roots of transgenic plants following RKN inoculation was observed within 16 h. Moreover, no increase in GUS expression after treatment with jasmonic acid was detected. Altogether, these results point to a putative role of this peroxidase in the general coffee response to RKN infection. (C) 2012 Elsevier Ireland Ltd. All rights reserved. (AU)