| Full text | |
| Author(s): |
Bressan, Eduardo de A.
[1]
;
Scotton, Danielle C.
[1]
;
Ferreira, Renato R.
[1]
;
Jorge, Erika C.
[2]
;
Sebbenn, Alexandre M.
[3]
;
Gerald, Lee T. S.
[4]
;
Figueira, Antonio
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Ctr Energia Nucl Agr, BR-13400970 Sao Paulo - Brazil
[2] Univ Fed Minas Gerais, Dept Morfol, BR-31270901 Belo Horizonte, MG - Brazil
[3] Secao Melhoramento & Conservaco Genet Florestal, Inst Florestal Sao Paulo, Sao Paulo - Brazil
[4] Univ Fed Sao Carlos, Ctr Ciencias Agr, BR-13600970 Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | AMERICAN JOURNAL OF BOTANY; v. 99, n. 6, p. E237-E239, JUN 2012. |
| Web of Science Citations: | 11 |
| Abstract | |
Premise of the study: Microsatellite primers were developed for Jatropha curcas (Euphorbiaceae), a tree species with large potential for biofuel production, to investigate its natural genetic diversity and mating system to facilitate the establishment of tree improvement and conservation programs. Methods and Results: Using a protocol for genomic library enrichment, 104 clones containing 195 repeat motifs were identified. Primer pairs were developed for 40 microsatellite loci and validated in 41 accessions of J. curcas from six provenances. Nine loci were polymorphic revealing from two to eight alleles per locus, and six primers were able to amplify alleles in the congeners J. podagrica, J. pohliana, and J. gossypifolia, but not in other Euphorbiaceae species, such as Hevea brasiliensis, Manihot esculenta, or Ricinus communis. Conclusions: The primers developed here revealed polymorphic loci that are suitable for genetic diversity and structure, mating system, and gene flow studies in J. curcas, and some congeners. (AU) | |
| FAPESP's process: | 07/04840-2 - Genetic variability and estimation of the outcrossing rate of the physic nut (Jatropha curcas L.) using molecular markers |
| Grantee: | Eduardo de Andrade Bressan |
| Support Opportunities: | Scholarships in Brazil - Doctorate |