| Full text | |
| Author(s): |
Colo Brunialti, Milena Karina
[1]
;
Santos, Michelle Carolina
[1]
;
Rigato, Otelo
[1, 2]
;
Machado, Flavia Ribeiro
[3]
;
Silva, Eliezer
[4]
;
Salomao, Reinaldo
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Fed Sao Paulo, Div Infect Dis, Dept Med, Escola Paulista Med, Hosp Sao Paulo, Sao Paulo - Brazil
[2] Hosp Sirio Libanes, Crit Care Unit, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Div Anesthesiol, Dept Surg, Escola Paulista Med, Hosp Sao Paulo, Sao Paulo - Brazil
[4] Hosp Albert Einstein, Crit Care Unit, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | PLoS One; v. 7, n. 5, p. e37393, 2012. |
| Web of Science Citations: | 11 |
| Abstract | |
Background: A shift from Th1 to Th2 as well as an increase in Treg CD4+T cell subsets has been reported in septic patients (SP). Furthermore, these patients display modulation of monocyte function, with reduced production of pro-inflammatory cytokines upon LPS stimulus, which resembles the phenotype of alternatively activated macrophages. In this study, we evaluated the percentages of T cells differentiated into Th1, Th17 and Treg subsets, as well as the percentage of monocytes expressing markers of alternatively activated monocytes/macrophages (AAM) in SP. Methodology/Principal Findings: Peripheral blood mononuclear cells (PBMC) were obtained from 32 healthy volunteers (HV) and from SP at admission (D0, n=67) and after 7 days of therapy (D7, n=33). Th1 and Th17 (CD3+CD8-) lymphocytes were identified by the intracellular detection of IFN-gamma and IL-17, respectively, spontaneously and after PMA/Io stimulation, and Treg cells were identified by Foxp3+CD127-expression. Monocytes were evaluated for CD206 and CD163 expression. Absolute numbers of CD4+T lymphocytes were measured in whole blood samples by flow cytometry. The Mann-Whitney or Wilcoxon test was applied, as appropriate. The percentage of Th1 cells was lower in SP than in HV at admission after PMA/Io stimulation, whereas the percentage of Th17 cells was higher. In patients' follow-up samples, a higher percentage of Th1 cells and a lower percentage of Th17 cells were observed on D7 compared with the D0 samples. Treg cells remained unchanged. Septic patients showed a markedly increased proportion of monocytes expressing CD163 and CD206. Conclusions/Significance: Upon in vitro stimulus, the percentage of T helper lymphocytes producing IL-17 was higher in SP than in HV at admission, and the percentage producing IFN-gamma was lower, a pattern that was reversed during follow-up. The increased expression of CD163 and CD206 indicates that monocytes may acquire the AAM phenotype during sepsis. (AU) | |
| FAPESP's process: | 06/58744-1 - Sepsis: integrating basic research and clinical investigation |
| Grantee: | Reinaldo Salomão |
| Support Opportunities: | Research Projects - Thematic Grants |