Activation of cytokines corroborate with development of inflammation and autoimmunity in thromboangiitis obliterans patients

Author(s)

Dellalibera-Joviliano, R. ^{[1, 2]} ; Joviliano, E. E. ^[1] ; Silva, J. S. ^[3] ; Evora, P. R. B. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Surg & Anat, BR-14048 Ribeirao Preto, SP - Brazil

^[2] Univ Ctr UNIFAFIBE, Bebedouro - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biochem & Immunol, Sao Paulo - Brazil

• Document type: Journal article
• Source: CLINICAL AND EXPERIMENTAL IMMUNOLOGY; v. 170, n. 1, p. 28-35, OCT 2012.
• Web of Science Citations: 15

Abstract

Thromboangiitis obliterans (TAO) is a segmental inflammatory occlusive disorder that affects the arm and leg arteries of young smokers. The immune system seems to play a critical role in the aetiology of TAO; however, knowledge of the aspects involved in the progression of vascular tissue inflammation and, consequently, the evolution of this disease is still limited. This study was carried out to investigate the cytokine levels of tumour necrosis factor (TNF)-a, interleukin (IL)-1 beta, IL-4, IL-17 and IL-23 in the plasma of TAO patients presenting with acute clinical manifestations. The study included 20 TAO patients (n = 10 women; n = 10 men) aged 3859 years under clinical follow-up, classified into two groups: (i) TAO former smokers (n = 11) and (ii) TAO active smokers (n = 9); the control groups included normal volunteer non-smokers (n = 10, active smokers (n = 10) and former smokers (n = 10). Patients' plasma samples were measured using the sandwich enzyme-linked immunosorbent assay. Statistical analyses were performed using the non-parametric MannWhitney U-test, with parameters significant at P < 0.05. The activities of all cytokines were different in groups of TAO patients when compared with normal controls, and decreased for control smokers. Increased levels of TNF-a, IL-1 beta, IL-4, IL-17 and IL-23 were significant in patients with TAO when compared to the controls (P < 0.005, all parameters). The results presented here indicate an increased production of cytokines in TAO, possibly contributing to the inflammatory response observed in the patients' vascular levels. In addition, the increased levels of IL-17 and IL-23 suggest that the disturbance of TAO is involved with mechanisms of autoimmunity. Thus, the discovery of IL-17 and its association with inflammation and autoimmune pathology has reshaped our viewpoint regarding the pathogenesis of TAO, which was based previously on the T helper type 1 (Th1)Th2 paradigm. (AU)