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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Occurrence and phylogenetic characterization of a baculovirus isolated from Culex quinquefasciatus in So Paulo State, Brazil

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Author(s):
da Cunha de Araujo Coutinho, Carlos Jose Pereira [1] ; Alves, Rafael [1] ; Sanscrainte, Neil D. ; Pinto Viviani, Andrea de Barros [2] ; dos Santos, Paulo Frugoli [2] ; Vasconcelos-Medeiros de Souza, Polyana A. [1] ; Vicente Guedes de Carvalho-Mello, Isabel Maria [1] ; Becnel, James J.
Total Authors: 8
Affiliation:
[1] Inst Butantan, Lab Imunol Viral, BR-05503900 Sao Paulo - Brazil
[2] Superintendencia Controle Endemias, BR-11665070 Caraguatatuba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ARCHIVES OF VIROLOGY; v. 157, n. 9, p. 1741-1745, SEP 2012.
Web of Science Citations: 3
Abstract

The aim of this study was to assess the occurrence of baculovirus infections in mosquitoes and characterize them by using molecular tools. Fortnightly collections were made of mosquito larvae in the city of Caraguatatuba. Six larvae of Culex quinquefasciatus were isolated that had white cysts (nodules) in epithelia cells of the posterior midgut, indicative of infection by a baculovirus. These larvae were subjected to DNA extraction. DNA was amplified, producing a fragment of around 600 nt of the lef-8 gene and 400 nt of Pif-2 gene. The sequences were aligned, using ClustalX 2.0, with partial sequences of lef-8 genes of baculoviruses isolated from members of other insect orders taken from the GenBank database and edited, and phylogenetic analysis was performed. Phylogenetic analysis performed with the lef-8 and pif-2 genes demonstrated that the baculovirus identified in Culex quinquefasciatus in the Caraguatatuba region is most closely related to the deltabaculovirus Culex nigripalpus nucleopolyhedrovirus. (AU)

FAPESP's process: 08/05700-2 - Entomopathogenic Microrganisms for Integrated Pest Management (IPM) of Diptera Insects: Molecular Characterization, Insect Pathology and Epidemiology
Grantee:Carlos José Pereira da Cunha de Araújo Coutinho
Support Opportunities: Regular Research Grants